Evidence map›Paper›PMID 41132063›Full record

ArticleAnimal bioscience2026

Effect of exogenous glucoamylase on ruminal in situ and in vitro dry matter and starch degradability of cereal grains in beef cattle.

Lin Mu, Kleves V Almeida, Carlos A NinodeGuzman, Ignacio F Marenchino, Kathy G Arriola, Halima Sultana, Nicolas DiLorenzo, Wenting Li, Diwakar Vyas

Abstract read
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In one paragraph

Article in Animal bioscience, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

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0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Lin MuDepartment of Grassland Science, College of Agronomy, Hunan Agricultural University, Changsha, China.
Kleves V AlmeidaCollege of Life Sciences and Agriculture, University of New Hampshire, Durham, NH, USA.
Carlos A NinodeGuzmanDepartment of Animal Sciences, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, FL, USA.
Ignacio F MarenchinoDepartment of Animal Sciences, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, FL, USA.
Kathy G ArriolaDepartment of Animal Sciences, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, FL, USA.
Halima SultanaDepartment of Animal Sciences, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, FL, USA.
Nicolas DiLorenzoDepartment of Animal Sciences, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, FL, USA.
Wenting LiDanisco Animal Nutrition and Health (IFF), Wilmington, DE, USA.
Diwakar VyasDepartment of Animal Sciences, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, FL, USA. diwakarvyas@ufl.edu.

Funding

Danisco Animal Nutrition and Health for this project
6 · The paper itself

Abstract

objectiveTo evaluate the effects of glucoamylase on ruminal in situ and in vitro dry matter degradability (IVDMD), in vitro starch degradability (IVSD), volatile fatty acids (VFA), gas production and methane production using cereal grains as substrates in beef cattle.

methodsSix substrates (4 mm; 0.70 g per F57 bag) including sorghum (micronized and whole), barley (whole and steam-flaked), and corn (dry-rolled and steam-flaked); were incubated with exogenous glucoamylase (from Trichoderma reesei; 0.25 mg/g substrate dry matter [DM]) and buffered rumen fluid in six replicates per run in 3 independent runs. IVDMD, IVSD, in situ DMD (ISDMD), VFA, and methane production was measured after 7, and 12 h of incubation. ISDMD was measured after 0, 1, 3, 7, and 12 h of ruminal incubation in beef cattle fed high grain diets.

resultsExogenous glucoamylase increased IVDMD and gas production for all substrates except whole sorghum and barley while ISDMD was increased for all substrates except whole barley. Glucoamylase increased IVSD for steam flaked barley and corn. Glucoamylase supplementation increased molar proportion of propionate and decreased acetate-to-propionate ratio, regardless of substrates used. Similarly, glucoamylase supplementation increased methane production with steam-flaked corn.

conclusionGlucoamylase supplementation can potentially improve DM and starch degradability in cereal grains commonly used as ingredients in beef cattle diets.

Indexed as

BeefGlucoamylaseIn Vitro Nutrient DegradabilityRumen FermentationVolatile Fatty Acids

Identifiers

PMID41132063
PMCPMC13547619

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.