Evidence map›Paper›PMID 41132060›Full record

ArticleAnimal bioscience2026

ELF5 modulates casein synthesis in goat mammary epithelial cells via JAK2/STAT5 signaling pathway.

Yuzhu Guo, Cunxia Ma, Tianxiang Meng, Tongtong Tu, Shuangshuang Cui, Yinghui Ling, Yunhai Zhang, Hongyu Liu, Ning Song

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PubMed Full text at PMC Publisher
In one paragraph

Article in Animal bioscience, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Yuzhu GuoAnhui Province Key Laboratory of Local Livestock and Poultry Genetic Resource Conservation and Germplasm Innovation, College of Animal Science and Technology, Anhui Agricultural University, Hefei, China.
Cunxia MaAnhui Province Key Laboratory of Local Livestock and Poultry Genetic Resource Conservation and Germplasm Innovation, College of Animal Science and Technology, Anhui Agricultural University, Hefei, China.
Tianxiang MengAnhui Province Key Laboratory of Local Livestock and Poultry Genetic Resource Conservation and Germplasm Innovation, College of Animal Science and Technology, Anhui Agricultural University, Hefei, China.
Tongtong TuAnhui Province Key Laboratory of Local Livestock and Poultry Genetic Resource Conservation and Germplasm Innovation, College of Animal Science and Technology, Anhui Agricultural University, Hefei, China.
Shuangshuang CuiAnhui Province Key Laboratory of Local Livestock and Poultry Genetic Resource Conservation and Germplasm Innovation, College of Animal Science and Technology, Anhui Agricultural University, Hefei, China.
Yinghui LingAnhui Province Key Laboratory of Local Livestock and Poultry Genetic Resource Conservation and Germplasm Innovation, College of Animal Science and Technology, Anhui Agricultural University, Hefei, China.
Yunhai ZhangAnhui Province Key Laboratory of Local Livestock and Poultry Genetic Resource Conservation and Germplasm Innovation, College of Animal Science and Technology, Anhui Agricultural University, Hefei, China.
Hongyu LiuAnhui Province Key Laboratory of Local Livestock and Poultry Genetic Resource Conservation and Germplasm Innovation, College of Animal Science and Technology, Anhui Agricultural University, Hefei, China.
Ning SongAnhui Province Key Laboratory of Local Livestock and Poultry Genetic Resource Conservation and Germplasm Innovation, College of Animal Science and Technology, Anhui Agricultural University, Hefei, China.

Funding

National Natural Science Foundation of China 32402718Talent Research Fund Project of Anhui Agricultural University rc392111
6 · The paper itself

Abstract

objectiveGoat milk contains a high concentration of caseins which are beneficial to human health. Understanding the regulatory mechanisms of casein synthesis in goats contribute to improving milk quality. Epithelial-specific E74-like factor 5 (ELF5) is important in milk protein synthesis, although the molecular mechanism by which ELF5 regulates casein synthesis remains unclear. This study aims to investigate the regulatory roles of ELF5 on casein production in goat mammary epithelial cells (GMECs).

methodsPrimary GMECs were isolated from goats and characterized using immunofluorescence and oil red O staining. The goat ELF5 gene overexpression vector and small interfering RNA were transfected into GMECs, respectively. Cell viability was accessed using cell counting kit-8 assay, and cell apoptosis was detected by flow cytometry. Genes involved in cell proliferation, apoptosis, and casein synthesis were examined by quantitative realtime polymerase chain reaction and Western blot. After co-treatment of Janus kinase 2 (JAK2) or signal transducer and activator of transcription 5 (STAT5) inhibitors, casein gene expression was examined by using Western blot. The interaction between ELF5 and STAT5 was verified by co-immunoprecipitation assay.

resultsELF5 enhances cell viability and the expression of genes associated with proliferation, while simultaneously inhibiting apoptosis and the expression of apoptosis-related genes. Then, ELF5 upregulates the expression of αS1-, αS2-, β- and κ-casein, in addition to enhancing JAK2/STAT5 signaling pathway. The inhibition assay of JAK2 and STAT5 activity reveals that ELF5 regulates casein synthesis via JAK2/STAT5 signaling pathway.

conclusionELF5 upregulates casein synthesis through the activation of the JAK2/STAT5 signaling pathway, offering a strategy for manipulating ELF5 to increase casein content and improve the protein quality of goat milk.

Indexed as

Casein SynthesisE74-like Factor 5Goat Mammary Epithelial CellsSignal Transducer and Activator of Transcription 5

Identifiers

PMID41132060
PMCPMC12877387

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