ArticleAnimal bioscience2026
Impact of chitosan supplementation on metabolomic profiles and microbial community dynamics in total mixed ration silage and rumen fluid.
Article in Animal bioscience, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
1 citing paper in PubMed.
- The impact of tomato pomace silage on feed utilization, health status, milk yield, and composition in dairy cows.Animal bioscience · 2026Article
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Authors and funding
7 authors.
Funding
Abstract
objectiveThis study aimed to investigate the effects of varying levels of chitosan supplementation in total mixed ration (TMR) silage on the abundance and dynamics of rumen microbial communities, as well as their associated metabolomic profiles.
methodsNext-generation sequencing and liquid chromatography-high resolution mass spectrometry-based metabolomics were employed to assess alterations in rumen microbiota and metabolites composition in response to chitosan supplementation in TMR silage.
resultsA total of 304 metabolites were identified in TMR silage, 144 of which had a variable importance in projection (VIP) scores greater than 1, marking them as distinguishing metabolites. Notably, chitosan supplementation increased L-valine levels, identifying it as a potential biomarker metabolite. In rumen fluid samples, 34 metabolites were identified, with 13 exhibiting VIP scores over 1, classifying them as key metabolite indicators. Chitosan supplementation significantly elevated amine compounds, particularly Dibenzylamine and N,N-Bis(2-hydroxyethyl) dodecanamide, in rumen fluid. The primary phyla affected by chitosan in TMR silage were Proteobacteria, Bacteroidota, and Firmicutes. Additionally, the genera Succinivibrionaceae_UCG-002 and Prevotella decreased with chitosan supplementation, whereas Rikenellaceae_RC9_gut_group exhibited increased abundance. Predominantly negative correlations were observed between rumen fluid metabolites (particularly amines and indoles) and microbial populations belonging to Bacteroidota and Firmicutes.
conclusionThese findings indicate that chitosan supplementation alters rumen metabolic activity and reduced microbial diversity within the rumen.
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Registered trials
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