Evidence map›Paper›PMID 41131031›Full record

ArticleScientific reports2025

Association of intermediate monocytes with dengue severity among the pediatric population of Odisha, India.

Manoj Kumar Dash, Sagnika Samal, Shailesh Rout, Rajesh Kumar Bhola, Janeka Gartia, Ishani Saha, Chinmay Kumar Behera, Hamida Hamdi, Mahesh Chandra Sahu, Biswadeep Das

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In one paragraph

Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Manoj Kumar DashSchool of Biotechnology, Kalinga Institute of Industrial Technology, Deemed to be University, Bhubaneswar, Odisha, 751024, India.
Sagnika SamalSchool of Biotechnology, Kalinga Institute of Industrial Technology, Deemed to be University, Bhubaneswar, Odisha, 751024, India.
Shailesh RoutSchool of Biotechnology, Kalinga Institute of Industrial Technology, Deemed to be University, Bhubaneswar, Odisha, 751024, India.
Rajesh Kumar BholaDepartment of Pathology, IMS & SUM Hospital, S`O`A Deemed to be University, Bhubaneswar, Odisha, 751003, India.
Janeka GartiaSchool of Biotechnology, Kalinga Institute of Industrial Technology, Deemed to be University, Bhubaneswar, Odisha, 751024, India.
Ishani SahaDepartment of Pediatrics, Kalinga Institute of Medical Sciences, KIIT-DU, Bhubaneswar, Odisha, 751024, India.
Chinmay Kumar BeheraDepartment of Pediatrics, Kalinga Institute of Medical Sciences, KIIT-DU, Bhubaneswar, Odisha, 751024, India.
Hamida HamdiDepartment of Biology, College of Science, Taif University, 21944, Taif, Saudi Arabia.
Mahesh Chandra SahuICMR-Regional Medical Research Centre, Bhubaneswar, Odisha, India. mcsahu@gmail.com.
Biswadeep DasSchool of Biotechnology, Kalinga Institute of Industrial Technology, Deemed to be University, Bhubaneswar, Odisha, 751024, India. biswadeep.das@kiitbiotech.ac.in.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Dengue virus (DENV) infection in children exhibits varied clinical presentations, wherein the role of monocytes is important in the innate immune response. In this study, laboratory-confirmed DENV pediatric patients (n = 120), with DENV-2 infection, were categorized into dengue fever (DF), dengue with warning signs (DWS) and severe dengue (SD) were assessed for monocyte subpopulation analysis using immunophenotyping involving CD14 and CD16 host-surface markers. Molecular docking was performed using HADDOCK 2.4 to analyze the interactions between CD14, CD16 and DENV envelope and capsid proteins. Among the cases, 84 (70%) were classified as DF and 36 (30%) as DWS & SD. Hematological and biochemical parameters indicated that thrombocytopenia and elevated hematocrit (> 40%) were significantly more common in DWS & SD, with markedly elevated liver enzymes (ALT and AST) in severe cases. Classical monocytes (CM-CD14++ CD16-) constituted 72.51% and 66.25% of the monocyte population in DF and DWS & SD cases, respectively. Intermediate monocytes (IM-CD14+ CD16+) comprised 9.89% and 30.86% in DF and DWS & SD cases, respectively. Non-classical monocytes (NCM-CD14+ CD16++) comprised 5.75% and 8.12% in DWS & SD and DF cases, respectively. In silico analysis revealed host CD16 and CD14 exhibited potential interactions with DENV capsid and envelope proteins, with binding energies - 8.9, - 10.1, - 8.6, and - 11.1 kcal/mol, respectively. IM was significantly increased in DWS & SD compared to DF (p < 0.05). These findings suggest that IM could act as host markers of DENV severity in children.

Indexed as

DengueDengue VirusMonocytesAdolescentCapsid ProteinsChildChild, PreschoolFemaleGPI-Linked ProteinsHumansImmunophenotypingIndiaInfantLipopolysaccharide ReceptorsMaleMolecular Docking SimulationCapsid ProteinsFCGR3B protein, humanGPI-Linked ProteinsLipopolysaccharide ReceptorsReceptors, IgGClassical monocytesDengue virusIntermediate monocytesMonocyte subpopulationsNon-classical monocytes

Identifiers

PMID41131031
PMCPMC12550026

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.