Evidence map›Paper›PMID 41130207›Full record

ArticleCell2025

Pan-modification profiling facilitates a cross-evolutionary dissection of the thermoregulated ribosomal epitranscriptome.

Miguel A Garcia-Campos, Joe Georgeson, Ronit Nir, Robert Reichelt, Kristin A Fluke, Donna Matzov, Vinithra Iyer, Brett W Burkhart, Lauren Lui, Anatoly Kustanovich and 21 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Seven wonders of RNA modification biology.RNA (New York, N.Y.) · 2026
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

31 authors.

Miguel A Garcia-CamposDepartment of Molecular Genetics, Weizmann Institute of Science, Rehovot 7610001, Israel.
Joe GeorgesonDepartment of Molecular Genetics, Weizmann Institute of Science, Rehovot 7610001, Israel.
Ronit NirDepartment of Molecular Genetics, Weizmann Institute of Science, Rehovot 7610001, Israel.
Robert ReicheltInstitute of Microbiology & Archaea Centre, Single-Molecule Biochemistry Lab, Regensburg Center for Biochemistry (RCB), University of Regensburg, 93053 Regensburg, Germany.
Kristin A FlukeDepartment of Biochemistry and Molecular Biology, Colorado State University, Fort Collins, CO 80523, USA.
Donna MatzovDepartment of Chemical and Structural Biology, Weizmann Institute of Science, Rehovot 7610001, Israel.
Vinithra IyerDepartment of Molecular Genetics, Weizmann Institute of Science, Rehovot 7610001, Israel.
Brett W BurkhartDepartment of Biochemistry and Molecular Biology, Colorado State University, Fort Collins, CO 80523, USA.
Lauren LuiMolecular Ecosystems Biology Department, Environmental Genomics and Systems Biology Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA.
Anatoly KustanovichDepartment of Molecular Genetics, Weizmann Institute of Science, Rehovot 7610001, Israel.
Felix GrünbergerInstitute of Microbiology & Archaea Centre, Single-Molecule Biochemistry Lab, Regensburg Center for Biochemistry (RCB), University of Regensburg, 93053 Regensburg, Germany.
Supuni Thalalla-GamageChemical Biology Laboratory, National Cancer Institute, Frederick, MD, USA.
Shereen A Howpay-ManageChemical Biology Laboratory, National Cancer Institute, Frederick, MD, USA.
Milan GerovacFaculty of Medicine, Institute for Molecular Infection Biology (IMIB), Helmholtz Institute for RNA-based Infection Research (HIRI), Helmholtz Centre for Infection Research (HZI), University of Würzburg, 97080 Würzburg, Germany.
Nicolas AlexandreRegensburg Centre for Biochemistry, Biochemistry III-Institute for Biochemistry, Genetics and Microbiology, University of Regensburg, Universitätsstraße 31, 93053 Regensburg, Germany.
Yuko NobeDepartment of Chemistry, Graduate School of Science, Tokyo Metropolitan University, Hachioji-shi, Tokyo 192-0397, Japan.
Jakub S NowakMalopolska Centre of Biotechnology, Jagiellonian University, 30-387 Krakow, Poland.
Manoj PereraDepartment of Chemistry and Health Research Institute, Michigan Technological University, 1400 Townsend Drive, Houghton, MI, USA.
Alexander ApostleDepartment of Chemistry and Health Research Institute, Michigan Technological University, 1400 Townsend Drive, Houghton, MI, USA.
Shiyue FangDepartment of Chemistry and Health Research Institute, Michigan Technological University, 1400 Townsend Drive, Houghton, MI, USA.
Sebastian GlattMalopolska Centre of Biotechnology, Jagiellonian University, 30-387 Krakow, Poland; Department of Biological Sciences and Pathobiology, University of Veterinary Medicine Vienna, 1210 Vienna, Austria.
Ghil JonaBacteriology & Genomic Repository Unit, Life Sciences Core Facilities, Weizmann Institute of Science, Rehovot 7610001, Israel.
Sébastien Ferreira-CercaRegensburg Centre for Biochemistry, Biochemistry III-Institute for Biochemistry, Genetics and Microbiology, University of Regensburg, Universitätsstraße 31, 93053 Regensburg, Germany; Laboratoire de Biologie Structurale de la Cellule (BIOC), UMR 7654-CNRS, École polytechnique, Institut Polytechnique de Paris, Route de Saclay, 91128 Palaiseau, France.
Jörg VogelFaculty of Medicine, Institute for Molecular Infection Biology (IMIB), Helmholtz Institute for RNA-based Infection Research (HIRI), Helmholtz Centre for Infection Research (HZI), University of Würzburg, 97080 Würzburg, Germany.
Masato TaokaDepartment of Chemistry, Graduate School of Science, Tokyo Metropolitan University, Hachioji-shi, Tokyo 192-0397, Japan.
Jordan L MeierChemical Biology Laboratory, National Cancer Institute, Frederick, MD, USA.
Eric WesthofArchitecture et Réactivité de l'ARN, Université de Strasbourg, Institut de biologie moléculaire et cellulaire du CNRS, Strasbourg 67084, France; Engineering Research Center of Clinical Functional Materials and Diagnosis & Treatment Devices of Zhejiang Province, Wenzhou Institute, University of Chinese Academy of Sciences, Wenzhou, China.
Thomas J SantangeloDepartment of Biochemistry and Molecular Biology, Colorado State University, Fort Collins, CO 80523, USA.
Dina GrohmannInstitute of Microbiology & Archaea Centre, Single-Molecule Biochemistry Lab, Regensburg Center for Biochemistry (RCB), University of Regensburg, 93053 Regensburg, Germany.
Moran Shalev-BenamiDepartment of Chemical and Structural Biology, Weizmann Institute of Science, Rehovot 7610001, Israel. Electronic address: moransb@weizmann.ac.il.
Schraga SchwartzDepartment of Molecular Genetics, Weizmann Institute of Science, Rehovot 7610001, Israel. Electronic address: schwartz@weizmann.ac.il.

Funding

Regulation of Archaeal TranscriptionR35GM143963 · NIGMS · COLORADO STATE UNIVERSITY · PI Thomas James Santangelo · 2022 to 2026
$1.8M
Synthesis of Sensitive Epitranscriptomically Modified RNAsR15GM109288 · NIGMS · MICHIGAN TECHNOLOGICAL UNIVERSITY · PI FANG, SHIYUE · 2014 to 2024
$1.3M
NIGMS NIH HHS R15 GM109288NIGMS NIH HHS R35 GM143963
6 · The paper itself

Abstract

Ribosomal RNA (rRNA) constitutes the core of ribosomes and is extensively chemically modified. Technical challenges have precluded systematically dissecting rRNA modifications and their dynamics. We develop Pan-Mod-seq, permitting inference of 16 distinct modifications across dozens of samples in parallel. We applied Pan-Mod-seq to RNA from 14 species spanning all domains of life, cultured under highly diverse conditions. While dynamic modifications are rare in mesophiles, in extreme hyperthermophiles, ∼50% of modifications are dynamic. We dissect the biogenesis and function of a conserved module of tandem m

Indexed as

RibosomesRNA, RibosomalTranscriptomeArchaeaCryoelectron MicroscopyEvolution, MolecularRNA Processing, Post-TranscriptionalTemperatureRNA, RibosomalepitranscriptomeribosomerRNA modifications

Identifiers

PMID41130207
PMCPMC12698195

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.