ArticleProtoplasma2026
Establishment of protoplast isolation, purification and transient transformation system from Rhododendron petals.
Article in Protoplasma, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
2 citing papers in PubMed.
- Haberlandt's dream and the secrets of the kitchen.Protoplasma · 2026Article
- An Efficient and Stable PEG-Mediated Transformation System for Medicinal FungusJournal of fungi (Basel, Switzerland) · 2026Article
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Authors and funding
8 authors.
Funding
Abstract
The genus Rhododendron, comprising approximately 1200 species, is the largest within its family and possesses significant ornamental value. However, functional genomics studies in this genus are hampered by an inefficient Agrobacterium-mediated stable transformation system. To facilitate rapid gene function verification, we developed a protoplast-based transient expression system using petal tissues. We optimized the enzymatic hydrolysis conditions (2.0% cellulase, 1.00% macerozyme, 0.6 mol/L mannitol, 8-h digestion) and established a two-step purification protocol involving centrifugal precipitation followed by flotation. For polyethylene glycol (PEG)-mediated transformation, the optimal parameters were 40% PEG4000, 0.10 mol/L CaCl
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Registered trials
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