Evidence map›Paper›PMID 41127871›Full record

ReviewCurrent research in insect science2025

Isolation of extracellular vesicles from insect hemolymph.

Stijn Van den Brande, Simon Remans, Anton Bilsen, Inge Corstjens, Evert Bruyninckx, Jozef Vanden Broeck, Dulce Santos

Abstract readReview
PubMed Full text at PMC Publisher
In one paragraph

Review in Current research in insect science, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Stijn Van den BrandeResearch group of Molecular Developmental Physiology and Signal Transduction, KU Leuven, Zoological Institute, Naamsestraat 59 box 2465, 3000 Leuven, Belgium.
Simon RemansResearch group of Molecular Developmental Physiology and Signal Transduction, KU Leuven, Zoological Institute, Naamsestraat 59 box 2465, 3000 Leuven, Belgium.
Anton BilsenResearch group of Molecular Developmental Physiology and Signal Transduction, KU Leuven, Zoological Institute, Naamsestraat 59 box 2465, 3000 Leuven, Belgium.
Inge CorstjensResearch group of Molecular Developmental Physiology and Signal Transduction, KU Leuven, Zoological Institute, Naamsestraat 59 box 2465, 3000 Leuven, Belgium.
Evert BruyninckxResearch group of Molecular Developmental Physiology and Signal Transduction, KU Leuven, Zoological Institute, Naamsestraat 59 box 2465, 3000 Leuven, Belgium.
Jozef Vanden BroeckResearch group of Molecular Developmental Physiology and Signal Transduction, KU Leuven, Zoological Institute, Naamsestraat 59 box 2465, 3000 Leuven, Belgium.
Dulce SantosResearch group of Molecular Developmental Physiology and Signal Transduction, KU Leuven, Zoological Institute, Naamsestraat 59 box 2465, 3000 Leuven, Belgium.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Extracellular vesicles (EVs) are produced by cells of all domains of life and current research shows their involvement in intercellular transfer of information. However, a major drawback to the progress of the field relates to the fact that isolating pure EV fractions from complex biofluids is a challenging task. Isolation of EVs is often compromised by the presence of contaminating protein and lipoprotein particles, which has recently led to the establishment of guidelines for analyzing and reporting on EVs. In insects, reports on EV studies are starting to emerge, with several techniques being used without consideration of contaminant co-isolation. To address this, we optimized and validated a robust procedure for the isolation of EVs from insect hemolymph.

Indexed as

ArgonauteDensity gradient ultracentrifugationDrosophila melanogasterLipophorin, Locusta migratoriaSize exclusion chromatography

Identifiers

PMID41127871
PMCPMC12538920

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.