Evidence map›Paper›PMID 41127630›Full record

ArticleOne health (Amsterdam, Netherlands)2025

Potential spillover investigated by metagenome sequencing in Laikipia, Kenya reveals tick-borne pathogens and a novel bunyavirus.

Maureen Kamau, Koray Ergunay, Brian P Bourke, Janerose Mutura, Rashid Lebunge, Griphin Ochieng, Kimita Gathii, John Waitumbi, Beth Mutai, James Hassell and 9 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in One health (Amsterdam, Netherlands), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

19 authors.

Maureen KamauMpala Research Centre, Nanyuki, Laikipia, Kenya.
Koray ErgunayDepartment of Entomology, Smithsonian Institution National Museum of Natural History, Washington, DC, USA.
Brian P BourkeDepartment of Entomology, Smithsonian Institution National Museum of Natural History, Washington, DC, USA.
Janerose MuturaMpala Research Centre, Nanyuki, Laikipia, Kenya.
Rashid LebungeMpala Research Centre, Nanyuki, Laikipia, Kenya.
Griphin OchiengMpala Research Centre, Nanyuki, Laikipia, Kenya.
Kimita GathiiKEMRI/WRAIR-Africa, Basic Science Laboratory, Kisumu Field Station, Nairobi, Kenya.
John WaitumbiKEMRI/WRAIR-Africa, Basic Science Laboratory, Kisumu Field Station, Nairobi, Kenya.
Beth MutaiKEMRI/WRAIR-Africa, Basic Science Laboratory, Kisumu Field Station, Nairobi, Kenya.
James HassellGlobal Health Program, Smithsonian's National Zoo and Conservation Biology Institute, Washington, DC, USA.
Michael E von FrickenGlobal Health Program, Smithsonian's National Zoo and Conservation Biology Institute, Washington, DC, USA.
Dawn ZimmermanDepartment of Entomology, Smithsonian Institution National Museum of Natural History, Washington, DC, USA.
Suzan MurrayGlobal Health Program, Smithsonian's National Zoo and Conservation Biology Institute, Washington, DC, USA.
Le JiangNaval Medical Research Center, Silver Spring, MD, USA.
Hsiao-Mei LiaoNaval Medical Research Center, Silver Spring, MD, USA.
John P GriecoDepartment of Biological Sciences, Eck Institute for Global Health, University of Notre Dame, Notre Dame, IN, USA.
Emily G McDermottDepartment of Entomology and Plant Pathology, University of Arkansas, Fayetteville, AR, USA.
Nicole L AcheeDepartment of Biological Sciences, Eck Institute for Global Health, University of Notre Dame, Notre Dame, IN, USA.
Yvonne-Marie LintonDepartment of Entomology, Smithsonian Institution National Museum of Natural History, Washington, DC, USA.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Tick-borne infections continue to present a global public health threat, and require a One Health approach for successful mitigation. We conducted cross-sectional tick screening utilizing an agnostic metagenomic screening strategy based on nanopore sequencing (NS), in an area spanning a range of habitats with intensified human-livestock-wildlife interactions in central Kenya. We further used targeted amplification by polymerase chain reaction (PCR) and sequence independent single primer amplification (SISPA) for confirmation and genome characterization, as necessary. We initially screened 44 ticks across pooled and individual samples belonging to seven species. Tick-associated bacteria-including spotted fever

Indexed as

BacteriaNanoporeScreeningSequencingSurveillanceTickVirus

Identifiers

PMID41127630
PMCPMC12538091

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.