Evidence map›Paper›PMID 41127269›Full record

ArticleHorticulture research2025

Systematic identification of terpene synthases from sacred lotus (

Zhenni Xu, Xueting Fang, Yao Zhi, Xiaochun Xiao, Jing Yang, Jie Hu, Hangzhi Zhu, Fangfang Chen, Weijia Cheng, Tiangang Liu and 1 more

Abstract read
In one paragraph

Article in Horticulture research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Terpene synthases screening in agarwood treeSynthetic and systems biotechnology · 2027
    Article
  2. Article
  3. Article
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Zhenni XuDepartment of Urology, Zhongnan Hospital of Wuhan University, Hubei Provincial Research Center for Basic Biological Science, School of Pharmaceutical Sciences, Wuhan University, Wuhan 430071, China.
Xueting FangDepartment of Urology, Zhongnan Hospital of Wuhan University, Hubei Provincial Research Center for Basic Biological Science, School of Pharmaceutical Sciences, Wuhan University, Wuhan 430071, China.
Yao ZhiDepartment of Urology, Zhongnan Hospital of Wuhan University, Hubei Provincial Research Center for Basic Biological Science, School of Pharmaceutical Sciences, Wuhan University, Wuhan 430071, China.
Xiaochun XiaoDepartment of Urology, Zhongnan Hospital of Wuhan University, Hubei Provincial Research Center for Basic Biological Science, School of Pharmaceutical Sciences, Wuhan University, Wuhan 430071, China.
Jing YangDepartment of Urology, Zhongnan Hospital of Wuhan University, Hubei Provincial Research Center for Basic Biological Science, School of Pharmaceutical Sciences, Wuhan University, Wuhan 430071, China.
Jie HuDepartment of Urology, Zhongnan Hospital of Wuhan University, Hubei Provincial Research Center for Basic Biological Science, School of Pharmaceutical Sciences, Wuhan University, Wuhan 430071, China.
Hangzhi ZhuDepartment of Urology, Zhongnan Hospital of Wuhan University, Hubei Provincial Research Center for Basic Biological Science, School of Pharmaceutical Sciences, Wuhan University, Wuhan 430071, China.
Fangfang ChenDepartment of Urology, Zhongnan Hospital of Wuhan University, Hubei Provincial Research Center for Basic Biological Science, School of Pharmaceutical Sciences, Wuhan University, Wuhan 430071, China.
Weijia ChengDepartment of Urology, Zhongnan Hospital of Wuhan University, Hubei Provincial Research Center for Basic Biological Science, School of Pharmaceutical Sciences, Wuhan University, Wuhan 430071, China.ORCID https://orcid.org/0009-0003-1499-5192
Tiangang LiuWuhan Hesheng Technology Co., Ltd., Wuhan 430074, China.
Li LuDepartment of Urology, Zhongnan Hospital of Wuhan University, Hubei Provincial Research Center for Basic Biological Science, School of Pharmaceutical Sciences, Wuhan University, Wuhan 430071, China.ORCID https://orcid.org/0000-0003-0490-7163

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Sacred lotus is widely used in the agricultural, nutraceutical, and pharmaceutical industries. Terpenes are not only crucial components of sacred lotus essential oil, but also serve as signaling molecules involved in plant-environment interactions. However, the biosynthesis of terpenes in sacred lotus has not yet been reported. Thus, gene-directed heterologous mining and combinatorial biosynthesis methods were used in this study to systematically characterize the function of terpene synthase genes in the sacred lotus. As a result, two monoterpene, 11 sesquiterpene, and three diterpene products were synthesized, and a highly efficient γ-eudesmol synthase was discovered. In addition, a mechanistic study revealed that N314 is the key amino acid responsible for the secondary cyclization that produces γ-eudesmol.

Identifiers

PMID41127269
PMCPMC12539867

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.