ArticleFrontiers in genome editing2025
CRISPR/Cas9- and Cas3-mediated modification of copy number variation in rice.
Article in Frontiers in genome editing, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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1 citing paper in PubMed.
- Dissecting Agronomically favorable genotypes in temperate japonica rice via haplotype analysis of a Japan-MAGIC population.The Plant journal : for cell and molecular biology · 2026Article
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5 authors.
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Abstract
Introduction: Copy number variation (CNV) is one of the crucial elements among genomic structural variations that span plant breeding. However, its impact on agricultural traits has remained elusive. Methods: We modulated CNVs using two genome-editing technologies, CRISPR/Cas9 and Cas3, along with their verification methods in rice to elucidate the effect of CNVs and further harness to improve relevant agronomic traits. Results: The addition of cytosine extension to the conventional single-guide RNA and its combination with Cas9 generated a frameshift mutation in parts of the Discussion: Altogether, the two technologies are expected to lay the foundation for new approaches to plant breeding by controlling CNV.
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