Evidence map›Paper›PMID 41126274›Full record

ArticleJournal of animal science and biotechnology2025

USP9X-triggered ferroptosis mediates follicular atresia via deubiquitinating Beclin1 in chicken.

Yuqi Chen, Wenjuan Wang, Can Cui, Yao Zhang, Zhuanjian Li, Huadong Yin, Shunshun Han

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Article in Journal of animal science and biotechnology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

7 authors.

Yuqi Chen *Key Laboratory of Livestock and Poultry Multi-Omics, Ministry of Agriculture and Rural Affairs, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China.
Wenjuan Wang *Key Laboratory of Livestock and Poultry Multi-Omics, Ministry of Agriculture and Rural Affairs, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China.
Can CuiKey Laboratory of Livestock and Poultry Multi-Omics, Ministry of Agriculture and Rural Affairs, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China.
Yao ZhangKey Laboratory of Livestock and Poultry Multi-Omics, Ministry of Agriculture and Rural Affairs, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China.
Zhuanjian LiCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou, 450002, Henan, China.
Huadong YinKey Laboratory of Livestock and Poultry Multi-Omics, Ministry of Agriculture and Rural Affairs, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China. yinhuadong@sicau.edu.cn.
Shunshun HanKey Laboratory of Livestock and Poultry Multi-Omics, Ministry of Agriculture and Rural Affairs, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China. hanshunshun@sicau.edu.cn.ORCID http://orcid.org/0000-0001-9394-1165

Funding

Agriculture Research System of China CARS-40National Key Research and Development Program of China 2022YFF1000202National Natural Science Foundation of China 32402745Sichuan Province Science and Technology Support Program 2021YFYZ0007Sichuan Province Science and Technology Support Program 2023NSFSC1940Sichuan Province Science and Technology Support Program 2024YFNH0025
6 · The paper itself

Abstract

backgroundFollicular atresia, a complex degenerative process regulated by multiple molecular mechanisms, significantly affects female reproductive performance in animals. While granulosa cell (GC) apoptosis has been well established as a primary mechanism underlying follicular atresia, the potential involvement of ferroptosis, which is an iron-dependent form of regulated cell death, remains largely unexplored in chickens.

resultsUsing a tamoxifen (TMX)-induced avian model of follicular atresia, we demonstrated that ferroptosis plays a critical role in follicular degeneration. Inhibition of ferroptosis through pharmacological agents significantly restored follicular function, underscoring its potential as a therapeutic target. Notably, we observed a significant upregulation of ubiquitin-specific peptidase 9, X-linked (USP9X) in GCs during atresia. Through comprehensive in vitro and in vivo investigations, we confirmed that USP9X facilitates follicular atresia by promoting ferroptosis in GCs. Mechanistically, USP9X induces ferroptosis by stabilizing Beclin1 through deubiquitination, thereby activating autophagy-dependent ferroptosis. This pathway was effectively suppressed by autophagy inhibitors, emphasizing the essential role of autophagy in USP9X-mediated ferroptosis.

conclusionsOur findings provide the evidence that the USP9X-Beclin1 axis regulates autophagy-dependent ferroptosis during avian follicular atresia. These insights reveal novel molecular targets and potential genetic markers for improving reproductive efficiency in chicken breeding programs.

Indexed as

AutophagyBeclin1FerroptosisFollicular atresiaUbiquitinationUSP9X

Identifiers

PMID41126274
PMCPMC12548185

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