ArticleJournal of animal science and biotechnology2025
Intramammary lipopolysaccharide infusion alters the fatty acid composition of blood lipid fractions but not milk in dairy cows.
Article in Journal of animal science and biotechnology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
backgroundMastitis is known to alter milk lipid yield, but its effects on lipid composition in blood and milk remain less understood. This study investigated changes in fatty acid (FA) composition in blood lipid fractions and milk of dairy cows following an intramammary lipopolysaccharide (LPS) challenge and explored potential links associated with these changes. We hypothesized that intramammary LPS infusion would alter the FA composition of blood lipid fractions, and that milk FA composition would reflect these changes. Furthermore, we hypothesized that prostaglandin E
resultsTen lactating cows were split into two groups. The treatment group received intramammary infusions of 50 μg Escherichia coli LPS in both quarters of one udder half to induce clinical mastitis, and saline infusions in the quarters of the opposite udder half; the control group received saline infusions in one udder half only. Blood and foremilk were collected from individual cows or glands at -1, 3, 6, 12, and 24 h post-infusion. Blood lipids were fractionated into cholesterol esters, free fatty acids (FFA), phospholipids (PL), and triacylglycerides (TAG). The FA composition was analyzed via gas-liquid chromatography. Total plasma TAG, FFA, and PGE
conclusionOur findings indicate that transient intramammary LPS challenge influences systemic lipid metabolism without altering the milk FA composition, suggesting that mammary inflammatory responses affect blood lipids independently of milk lipid secretion.
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