Evidence map›Paper›PMID 41126094›Full record

ArticleBMC biotechnology2025

Interaction of potato-derived RG-I with galectin-3 and gene regulation in human mesenchymal stem cells.

Amir Mukhtar, Athina Giannoudis, Bodil Jørgensen, Dongmei Wu, Xuan Liu, Kamal Babikeir Elnour Mustafa, Gudveig Cecilie Gjerde Gjengedal, Oluwatobi Adegbite, Anna Mieszkowska, Lu-Gang Yu and 1 more

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Article in BMC biotechnology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

11 authors.

Amir MukhtarInstitute of Life Course and Medical Sciences, School of Dentistry, University of Liverpool, Liverpool, UK.
Athina GiannoudisInstitute of Life Course and Medical Sciences, School of Dentistry, University of Liverpool, Liverpool, UK.
Bodil JørgensenDepartment of Plant and Environmental Sciences, University of Copenhagen, Copenhagen, Denmark.
Dongmei WuDepartment of Plant and Environmental Sciences, University of Copenhagen, Copenhagen, Denmark.
Xuan LiuShared Research Facilities, Centre for Genomic Research, University of Liverpool, Liverpool, UK.
Kamal Babikeir Elnour MustafaDepartment of Clinical Dentistry, University of Bergen, Bergen, NO-5020, Norway.
Gudveig Cecilie Gjerde GjengedalDepartment of Clinical Dentistry, University of Bergen, Bergen, NO-5020, Norway.
Oluwatobi AdegbiteInstitute of Systems, Molecular and Integrative Biology, University of Liverpool, Liverpool, UK.
Anna MieszkowskaDepartment of Evolutionary Immunology, Institute of Zoology and Biomedical Research, Faculty of Biology, Jagiellonian University, Krakow, Poland.
Lu-Gang YuInstitute of Systems, Molecular and Integrative Biology, University of Liverpool, Liverpool, UK.
Katarzyna Gurzawska-ComisInstitute of Life Course and Medical Sciences, School of Dentistry, University of Liverpool, Liverpool, UK. kasia.gurzawska-comis@dent.au.dk.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundMesenchymal stem cells (MSC) play a vital role in bone regeneration. Poly-(l-lactide-co-ε-caprolactone) scaffolds functionalised with modified rhamnogalacturonan-I (RG-I) potato pectin, can modulate inflammation and promote bone regeneration in-vitro and in-vivo by modulating the action of the galactoside-binding galectin-3. In this study, we determined the binding affinity to galectin-3 of potato unmodified RG-I (PU) and its arabinose-deficient form (PA) and investigated the transcriptomic effects of PA treatment on human MSCs evaluated through in-silico pathway analysis in relation to galectin-3.

methodsThe binding affinity of RG-I (PU and PA) to galectin-3 was assessed by tryptophan fluorescence spectroscopy. Human MSC (hMSC) isolated from the bone marrow of elderly patients (> 60yrs), were analysed by transcriptomic profiling. The effect of PA surface coating on gene expression was assessed in-silico by the ingenuity pathway analysis (IPA).

resultsRG-I binds to galectin-3 with a binding affinity of 8.66 × 10

conclusionsThis study indicates that modified potato pectin (PA) can regulate the expression of a broad range of genes, including galectin-3, that are involved in cellular, inflammatory, and immunological functions of hMSCs and may potentially be used to promote bone regeneration via modulation of inflammation.

Indexed as

Galectin 3Gene Expression RegulationMesenchymal Stem CellsPectinsSolanum tuberosumBlood ProteinsCells, CulturedGalectinsGene Expression ProfilingHumansBlood ProteinsGalectin 3GalectinsLGALS3 protein, humanPectinsBone regenerationGalectin-3Inflammatory responseIn-silico analysisPlant-derived nanoparticlesRhamnogalacturonan-I (RG-I)Transcriptomic profiling

Identifiers

PMID41126094
PMCPMC12548138

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.