ArticleBMC microbiology2025
Whole-genome sequencing reveals resistance mechanisms and molecular epidemiology of carbapenem-resistant Pseudomonas aeruginosa bloodstream infections.
Article in BMC microbiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- Comparative genomic analysis of clinical Pseudomonas aeruginosa isolates from Iraq: insights into genome diversity, antimicrobial resistance, and phylogenetic relatedness.Molecular biology reports · 2026Article
- Efflux-mediated carbapenem resistance: unveiling genetic drivers, clinical implications, and strategies for global antimicrobial stewardship.Antonie van Leeuwenhoek · 2026Review
- Genomic Analysis of Ocular Pseudomonas aeruginosa Isolates: Insights From a Predominantly Asian, Multi-Regional Dataset.Investigative ophthalmology & visual science · 2026Article
- First reported Pseudomonas soli bloodstream infection confirmed by whole-genome sequencing after MALDI-TOF misidentification.BMC microbiology · 2026Article
- Genomic insights into antimicrobial resistance in ocular pathogens from India.Communications biology · 2026Article
- Predictors of Mortality inPathogens (Basel, Switzerland) · 2026Review
- Distribution and Antimicrobial Resistance Trends of Bloodstream Bacterial Isolates: A 10-year Single-Center Study in China.Infection and drug resistance · 2026Article
- Impact of a Pharmacist-Led Grid-Based Stewardship Program on the Association Between Antimicrobial Use and Resistance: A 7.5-Year Interrupted Time-Series Analysis in a Chinese Tertiary Hospital.Infection and drug resistance · 2026Article
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Abstract
backgroundCarbapenem-resistant Pseudomonas aeruginosa (CRPA) has emerged as a critical threat in bloodstream infections (BSIs), with rising global prevalence and elevated mortality rates. Traditional surveillance methods often lacks resolution for resistance-virulence-transmission interplay, highlighting the importance of high-resolution genomics. Whole-genome sequencing (WGS) has enabled unprecedented resolution in dissecting CRPA's genetic landscape, revealing links between resistance, virulence, and outcomes.
resultsThis study employed WGS to characterize 61 P. aeruginosa isolates from BSIs, with a focus on 18 CRPA strains. Clinical data linked central venous catheterization to CRPA BSI development (OR = 6.6, p = 0.002) and identified carbapenem exposure, mechanical ventilation, and low hemoglobin as independent mortality risk factors. WGS identified 33.3% (n = 6, 6/18) of the strains harbored β-lactamase genes, and 44.4%(n = 8, 8/18) of the strains carried truncated OprD protein due to frameshift mutations or point mutations inducing translational truncation. Efflux pump overexpression (61.1% with ≥ 2-fold upregulation) further contributed to this resistance phenotype. MLST identified 49 distinct STs (including 2 novel types) and a pattern of endemic diversification. O11 is strongly linked to carbapenem resistance (CRPA: p = 0.02; MDRPA: p = 0.004), correlating with oprD mutations (p = 0.008) and exoU+/exoS-, indicating enhanced nosocomial adaptability.
conclusionsA very high genetic diversity was noted amongst P. aeruginosa strains isolated from BSIs cases. The mechanism of carbapenem resistance is mainly attributed to oprD mutations and efflux pumps activation, with carbapenemases emerging as an additional mechanism of concern. These resistance mechanisms with high-risk clinical factors collectively indicate that strict policies are essential for in CRPA BSIs management.
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