ArticleInternational microbiology : the official journal of the Spanish Society for Microbiology2025
Optimisation of physicochemical factors for enhanced astaxanthin accumulation in newly isolated freshwater green microalgae Desmodesmus sp. PLM2: insights from current analytical techniques.
Article in International microbiology : the official journal of the Spanish Society for Microbiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Microalgae are a rich source of valuable products like astaxanthin, a health-promoting ketocarotenoid, but their carotenoid production is challenging due to demanding culture conditions. Consequently, an attempt was made to isolate and screen a new microalgae strain for astaxanthin production via a two-stage cultivation method. In the first stage (vegetative phase), a nutrient-rich medium was used to promote cell growth and biomass, and in the second stage, astaxanthin accumulation was stimulated by stress conditions (0.2% NaCl and 4.4 mM sodium acetate) with continuous light illumination. The effect of physicochemical factors on cell growth, chlorophyll, and carotenoid content was intensively investigated. The microalgal strain was genomically identified as Desmodesmus sp. PLM2 using 18S rRNA sequencing. Optimised conditions, i.e. 20 mM sodium nitrate, 3 mM glucose, 32 mM potassium chloride, pH 7, and temperature 27 °C yielded a maximum biomass of 4.7 g/L, total chlorophyll content of 27.13 µg mL
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