Evidence map›Paper›PMID 41125734›Full record

ArticleScientific reports2025

Evaluation of cationic peptide-based nanogels as delivery systems for negatively charged molecules: a formulative study.

Mariangela Rosa, Elisabetta Rosa, Valeria Castelletto, Ian W Hamley, Giancarlo Morelli, Antonella Accardo, Carlo Diaferia

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Mariangela RosaDepartment of Pharmacy and Interuniversity Research Centre on Bioactive Peptides "Carlo Pedone" (CIRPeB), , University of Naples "Federico II", Via Tommaso De Amicis 95, Naples, 80145, Italy.
Elisabetta RosaDepartment of Pharmacy and Interuniversity Research Centre on Bioactive Peptides "Carlo Pedone" (CIRPeB), , University of Naples "Federico II", Via Tommaso De Amicis 95, Naples, 80145, Italy.
Valeria CastellettoDepartment of Pharmacy and Interuniversity Research Centre on Bioactive Peptides "Carlo Pedone" (CIRPeB), , University of Naples "Federico II", Via Tommaso De Amicis 95, Naples, 80145, Italy.
Ian W HamleySchool of Chemistry, Pharmacy and Food Biosciences, University of Reading, RG6 6AD, Berkshire, UK.
Giancarlo MorelliDepartment of Pharmacy and Interuniversity Research Centre on Bioactive Peptides "Carlo Pedone" (CIRPeB), , University of Naples "Federico II", Via Tommaso De Amicis 95, Naples, 80145, Italy.
Antonella AccardoDepartment of Pharmacy and Interuniversity Research Centre on Bioactive Peptides "Carlo Pedone" (CIRPeB), , University of Naples "Federico II", Via Tommaso De Amicis 95, Naples, 80145, Italy.
Carlo DiaferiaDepartment of Pharmacy and Interuniversity Research Centre on Bioactive Peptides "Carlo Pedone" (CIRPeB), , University of Naples "Federico II", Via Tommaso De Amicis 95, Naples, 80145, Italy. carlo.diaferia@unina.it.

Funding

Ministero dell'Università e della Ricerca PRIN_2022TSLMHRNational Center for Gene Therapy and Drugs based on RNA Technology CN00000041
6 · The paper itself

Abstract

Peptide-based nanogels (NGs) represent a cutting-edge class of nanoscale drug delivery systems. Due to their structural properties, NGs platforms can encapsulate and protect therapeutic agents (e.g. peptides, proteins, and nucleic acids), while allowing for controlled and stimuli-responsive release. These pharmacokinetic and pharmacodynamic features can be specifically tuned by including peptide functional elements as NG components. This study explores the formulation, decoration strategies, and structural properties of NGs derived from mixed hydrogel (HG) matrices of Fmoc-diphenylalanine (Fmoc-FF) with cationic amphiphilic peptides (CAPs). CAPs, composed by cationic hexapeptide (GK)3 sequence decorated at its N-terminus with alkyl chain, were found able to confer a net positive charge to Fmoc-FF NGs. Fmoc-FF/C16-(GK)3 and Fmoc-FF/C18-(GK)3 NGs were obtained using polysorbate 80 (TWEEN 80) and sorbitane monooleate 80 (SPAN 80) as colloidal stabilizing surfactants and characterized in terms of size, secondary structure, superficial charge and shelf stability by Dynamic Light Scattering (DLS), Circular Dichroism (CD), Fourier Transform Infrared (FT-IR) and Small-Angle X-ray Scattering (SAXS) technique. Different formulative routes were used and mutually compared to encapsulate or adsorb AlexaFluor 430 (succinimidyl ester), used as model of an anionic, pharmaceutical agent. In vitro experiments demonstrated good cytocompatibility of these systems and the release of AlexaFluor 430 was also evaluated.

Indexed as

Drug Delivery SystemsNanogelsPeptidesCationsDrug CarriersHumansHydrogelsSpectroscopy, Fourier Transform InfraredCationsDrug CarriersHydrogelsNanogelsPeptidesAnionic active pharmaceutical ingredientsDrug deliveryFormulation studyPeptide-based nanoplatformsPeptide nanogels

Identifiers

PMID41125734
PMCPMC12546590

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.