ArticleUltrasonics sonochemistry2025
Unveiling the efficiency of pulsed electric field and ultrasonication in enhancing collagen susceptibility to enzymatic hydrolysis.
Article in Ultrasonics sonochemistry, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Since collagen has naturally evolved to be durable, producing collagen hydrolysate with low molecular weight (LMW) peptides which typically results in high bioactivity is a challenge. Here, the efficiency of ultrasound (US) and pulsed electric field (PEF) pretreatments in enhancing the enzymatic hydrolysis of starfish collagen biomass (SFCB) and the antioxidant capacity of the resulting hydrolysate were evaluated. Both pretreatments significantly improved the degree of hydrolysis (DH) and reduced the required hydrolysis time compared to the conventional methods. The DH of SFCB hydrolysate pretreated with US reached 20 % at 60 min while that of the PEF treatment reached 21 % at 120 min, matching the DH of the conventional hydrolysis at 240 min. US and PEF hereby increased the collagen hydrolysate recovery, particularly compensating for a low enzyme-to-substrate ratio (0.5 % v/w). The pretreatments resulted in collagen peptides with significantly higher antioxidant properties, as assessed by ABTS and DPPH assays. The IC50 values of ABTS radical inhibition were found to be 0.68, 0.43, and 0.84 mg/ml for the collagen peptides produced by the US, PEF pretreatments and conventional enzymatic hydrolysis, respectively. Applying PEF and US pretreatment at an enzyme concentration of 0.5 % v/w, increased the proportion of collagen hydrolysate with MW < 10 KDa to 64 % and 59 %, respectively, compared to the conventional method which yielded only 43 %. The enhancements in the antioxidant activities were linked to the increased proportion of LMW peptides (<10 kDa) and elevated relative levels of antioxidative amino acids e.g. glycine, proline, basic amino acids, and hydrophobic amino acids in the peptides produced using US and PEF pretreatment. Overall, US and PEF pretreatments effectively increased collagen susceptibility to enzymatic hydrolysis, improving efficiency and generating peptides with superior antioxidant properties.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.