ArticleBrazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologica2025
Establishing the reference intervals for subpopulations of T lymphocytes, B lymphocytes, and natural killer cells: insight from the Brazilian Longitudinal Study of Adult Health (ELSA-Brasil).
Article in Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologica, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The aim of this study was to establish reference intervals for lymphocyte subpopulations in the peripheral blood of Brazilian adults, and to assess potential variations by gender and age groups. The study assessed 351 healthy participants of the Brazilian Longitudinal Study of Adult Health (ELSA-Brasil). Lymphocyte subpopulations were analyzed by dual platform using automated hematological analyzer Sysmex XN-3000 and the four-color flow cytometer on the FACSCalibur. Reference intervals were established using the 2.5th and 97.5th percentiles. Z-score was employed to ascertain the need for distinct reference intervals across gender and age groups. The Mann-Whitney test, with a significance level set at P<0.05, was conducted to identify differences among population groups. The absolute and relative reference intervals were: total lymphocytes: 1.0-2.9 (×103/µL); CD3+: 721.0-2311.5 cells/μL, 59.1-84.5%; CD4+: 421.4-1523.8 cells/μL, 32.5-61.6%; CD8+: 175.3-879.8 cells/μL, 12.3-39.1%; CD4+CD8+: 0.8-4.1 cells/μL; CD19+: 85.2-501.6 cells/μL, 5.6-21.0%; NK: 83.9-444.4 cells/μL, 4.3-23.5%. Significant gender and age differences were observed in both the relative and absolute values of most lymphocyte subpopulations. There are variations in lymphocyte subsets across the global population, underscoring the need to establish tailored reference intervals for distinct populations, particularly for Helper T lymphocytes, B lymphocytes, and NK cells.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.