Evidence map›Paper›PMID 41124422›Full record

ArticleJournal of applied oral science : revista FOB2025

Comprehensive analysis of transcriptome and pathway interactions in periodontitis.

Bruno César de Vasconcelos Gurgel, Nathalia Vilela, Kaio Henrique Soares, Luiz Eduardo Rodrigues Juliasse, Ikramuddin Aukhil, Poliana Mendes Duarte

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Article in Journal of applied oral science : revista FOB, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Bruno César de Vasconcelos GurgelUniversidade Federal do Rio Grande do Norte, Departamento de Odontologia, Natal, RN, Brasil.ORCID http://orcid.org/0000-0001-5971-134X
Nathalia VilelaUniversidade de São Paulo, Faculdade de Odontologia, Departamento de Estomatologia, Divisão de Periodontia, São Paulo, SP, Brasil.ORCID http://orcid.org/0000-0002-0111-2309
Kaio Henrique SoaresUniversidade Federal dos Vales do Jequitinhonha e Mucuri, Departamento de Odontologia, Diamantina, MG, Brasil.ORCID http://orcid.org/0000-0002-1239-9296
Luiz Eduardo Rodrigues JuliasseUniversidade Federal do Rio Grande do Norte, Departamento de Odontologia, Natal, RN, Brasil.ORCID http://orcid.org/0009-0009-9697-4777
Ikramuddin AukhilEast Carolina University, ECU School of Dental Medicine, Greenville, NC, USA.ORCID http://orcid.org/0000-0002-7127-3693
Poliana Mendes DuarteUniversity of Florida College of Dentistry, Department of Periodontology, Gainesville, FL, USA.ORCID http://orcid.org/0000-0001-8872-5943

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveTo investigate the transcriptomic profile and dysregulated molecular pathways associated with severe periodontitis. METHODOLOGY: Gingival tissues from patients with severe periodontitis (n=11) and periodontally healthy controls (n=11) were compared using RNA sequencing in this cross-sectional study. Differentially expressed genes (DEGs) were identified and analyzed using Ingenuity Pathway Analysis (IPA). Selected DEGs were validated at the protein level via immunohistochemistry (IHC).

resultsA total of 909 DEGs were upregulated and 742 were downregulated in periodontitis versus healthy tissues. Highly upregulated genes included MT-RNR1, MTRNR2L12, pseudogenes, long non-coding RNAs, and immunoglobulins. Downregulated genes included ADGRG7, C6orf15, members of enzyme families, keratin family members, loricrin (LOR), and immune modulators, such as CD207 (Langerin) and DEFB4A. IPA predicted the Wnt/β-catenin pathway as the most upregulated and the IL-17 signaling pathway as the most suppressed canonical pathway in periodontitis. The expression of the level of protein of LOR, Wnt10b, JUN, and FOS was confirmed by IHC.

conclusionDysregulation in key canonical signaling pathways and the altered expression of genes critical to cell chemotaxis, innate immunity, and epithelial barrier integrity seem to play a pivotal role in the pathogenesis of periodontitis.

Indexed as

PeriodontitisTranscriptomeAdultCase-Control StudiesCross-Sectional StudiesDown-RegulationFemaleGene Expression ProfilingGingivaHumansImmunohistochemistryMaleMiddle AgedReference ValuesReproducibility of ResultsSequence Analysis, RNA

Identifiers

PMID41124422
PMCPMC12677198

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.