Evidence map›Paper›PMID 41123970›Full record

ArticleSmall (Weinheim an der Bergstrasse, Germany)2025

Automated Covalent Microcontact Bioprinting of Lubricant-Infused Microarrays for Biological Assays.

Lubna Najm, Amid Shakeri, Fereshteh Bayat, Shaghayegh Moghimikandelousi, Akansha Prasad, Liane Ladouceur, Samantha Dacalos, Sakina Hussain, Inaam Chattha, Hareet Sidhu and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Small (Weinheim an der Bergstrasse, Germany), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Lubna NajmDepartment of Mechanical Engineering, McMaster University, 1280 Main Street West, Hamilton, Ontario, L8S 4L7, Canada.ORCID 0009-0009-7371-2125
Amid ShakeriInstitute of Biomedical Engineering, University of Toronto, Toronto, Ontario, M5S 3G9, Canada.ORCID 0000-0002-4534-382X
Fereshteh BayatDepartment of Mechanical Engineering, McMaster University, 1280 Main Street West, Hamilton, Ontario, L8S 4L7, Canada.ORCID 0000-0002-8868-1650
Shaghayegh MoghimikandelousiSchool of Biomedical Engineering, McMaster University, 1280 Main Street West, Hamilton, Ontario, L8S 4L7, Canada.
Akansha PrasadSchool of Biomedical Engineering, McMaster University, 1280 Main Street West, Hamilton, Ontario, L8S 4L7, Canada.ORCID 0000-0002-2636-5097
Liane LadouceurDepartment of Mechanical Engineering, McMaster University, 1280 Main Street West, Hamilton, Ontario, L8S 4L7, Canada.ORCID 0000-0002-7139-8162
Samantha DacalosDepartment of Biochemistry and Biomedical Sciences, McMaster University, 1280 Main Street West, Hamilton, Ontario, L8S 4K1, Canada.
Sakina HussainDepartment of Biochemistry and Biomedical Sciences, McMaster University, 1280 Main Street West, Hamilton, Ontario, L8S 4K1, Canada.ORCID 0009-0004-0575-823X
Inaam ChatthaDepartment of Biochemistry and Biomedical Sciences, McMaster University, 1280 Main Street West, Hamilton, Ontario, L8S 4K1, Canada.ORCID 0009-0001-6123-9264
Hareet SidhuDepartment of Biochemistry and Biomedical Sciences, McMaster University, 1280 Main Street West, Hamilton, Ontario, L8S 4K1, Canada.ORCID 0009-0002-3343-1889
Zeinab HosseinidoustSchool of Biomedical Engineering, McMaster University, 1280 Main Street West, Hamilton, Ontario, L8S 4L7, Canada.ORCID 0000-0001-9261-7611
Tohid F DidarDepartment of Mechanical Engineering, McMaster University, 1280 Main Street West, Hamilton, Ontario, L8S 4L7, Canada.ORCID 0000-0002-8757-8002

Funding

Natural Sciences and Engineering Research Council of CanadaOntario Ministry of Colleges and Universities
6 · The paper itself

Abstract

Microcontact bioprinting (µCP), which utilizes elastomeric stamps to transfer biorecognition agents (bioinks) onto substrates, offers advantages such as customizability, cost-effectiveness, and versatility for bioassays. Despite its prevalent use in laboratory settings, µCP faces challenges in achieving the repeatability and reproducibility required for industrial applications. Here, a µCP method is introduced that accommodates the immobilization of various biorecognition agents, while preserving bioactivity for use in bioassays. A key innovation lies in combining µCP with fluorosilanization to enable lubricant-infused surfaces that prevent non-specific attachment, while concurrently enhancing biomolecule immobilization via covalent attachment through a modified bioink formulation. Furthermore, an automated µCP system is integrated, taking critical steps toward industrial scalability. Following optimization with fluorescent proteins, bacterial viruses (bacteriophages) are printed. The bioactivity preservation is confirmed using Pseudomonas aeruginosa bacteriophage microarrays. A bacterial metabolic activity bioassay is conducted, whereby bacteriophage lytic activity led to a visible color difference after 3 h. The introduced µCP is high-throughput, scalable, and highly customizable, demonstrating strong potential for industrial implementation.

Indexed as

Biological AssayBioprintingLubricantsMicroarray AnalysisAutomationBacteriophagesPseudomonas aeruginosaLubricantsautomationbacteriophagefailure mode and effects analysislubricant‐infused surfacesmicroarraysmicrocontact bioprinting

Identifiers

PMID41123970
PMCPMC12696803

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.