Evidence map›Paper›PMID 41123686›Full record

ArticleTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik2025

Molecular dissection of large-spike trait in wheat revealing stable QTLs and REGULATOR OF GRAIN NUMBER1 as targets for yield improvement.

Yujie Jiang, Haoran Wang, Yanming Ma, Junxian Liu, Zhaoyan Chen, Chaoqun Dong, Xiangqing Liu, Xincheng Wang, Yiqing Wang, Sufang Li and 7 more

Abstract read
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In one paragraph

Article in TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

17 authors.

Yujie JiangState Key Laboratory of High-Efficiency Production of Wheat-Maize Double Cropping, Frontiers Science Center for Molecular Design Breeding, China Agricultural University, Beijing, 100193, China.
Haoran WangState Key Laboratory of High-Efficiency Production of Wheat-Maize Double Cropping, Frontiers Science Center for Molecular Design Breeding, China Agricultural University, Beijing, 100193, China.
Yanming MaCrop Research Institute of Xinjiang Uygur Autonomous Region Academy of Agricultural Sciences, National Central Asian Characteristic Crop Germplasm Resources Medium-term Gene Bank, Urumqi, 830091, China.
Junxian LiuState Key Laboratory of High-Efficiency Production of Wheat-Maize Double Cropping, Frontiers Science Center for Molecular Design Breeding, China Agricultural University, Beijing, 100193, China.
Zhaoyan ChenInstitute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
Chaoqun DongState Key Laboratory of High-Efficiency Production of Wheat-Maize Double Cropping, Frontiers Science Center for Molecular Design Breeding, China Agricultural University, Beijing, 100193, China.
Xiangqing LiuState Key Laboratory of High-Efficiency Production of Wheat-Maize Double Cropping, Frontiers Science Center for Molecular Design Breeding, China Agricultural University, Beijing, 100193, China.
Xincheng WangState Key Laboratory of High-Efficiency Production of Wheat-Maize Double Cropping, Frontiers Science Center for Molecular Design Breeding, China Agricultural University, Beijing, 100193, China.
Yiqing WangState Key Laboratory of High-Efficiency Production of Wheat-Maize Double Cropping, Frontiers Science Center for Molecular Design Breeding, China Agricultural University, Beijing, 100193, China.
Sufang LiState Key Laboratory of High-Efficiency Production of Wheat-Maize Double Cropping, Frontiers Science Center for Molecular Design Breeding, China Agricultural University, Beijing, 100193, China.
Yingyin YaoState Key Laboratory of High-Efficiency Production of Wheat-Maize Double Cropping, Frontiers Science Center for Molecular Design Breeding, China Agricultural University, Beijing, 100193, China.
Mingming XinState Key Laboratory of High-Efficiency Production of Wheat-Maize Double Cropping, Frontiers Science Center for Molecular Design Breeding, China Agricultural University, Beijing, 100193, China.
Zhongfu NiState Key Laboratory of High-Efficiency Production of Wheat-Maize Double Cropping, Frontiers Science Center for Molecular Design Breeding, China Agricultural University, Beijing, 100193, China.
Huiru PengState Key Laboratory of High-Efficiency Production of Wheat-Maize Double Cropping, Frontiers Science Center for Molecular Design Breeding, China Agricultural University, Beijing, 100193, China.
Qixin SunState Key Laboratory of High-Efficiency Production of Wheat-Maize Double Cropping, Frontiers Science Center for Molecular Design Breeding, China Agricultural University, Beijing, 100193, China.
Xingguo YeInstitute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
Jie LiuState Key Laboratory of High-Efficiency Production of Wheat-Maize Double Cropping, Frontiers Science Center for Molecular Design Breeding, China Agricultural University, Beijing, 100193, China. jieliu@cau.edu.cn.ORCID http://orcid.org/0000-0002-1415-3847

Funding

National Key Research and Development Program of China 2022YFF1003401National Major Science and Technology Projects of China 2023ZD0402402National Natural Science Foundation of China 32372120
6 · The paper itself

Abstract

key messageThis study reveals a pleiotropic QTL QPh/Sl/Sn.cau-2D.1 coordinating plant architecture and spike morphology in wheat, and identifies REGULATOR OF GRAIN NUMBER1 (TaRGN-D1) as a key regulator of spikelet number, providing molecular targets and diagnostic markers for yield improvement. Spike architecture traits, particularly spike length (SL) and spikelet number per spike (SN), are crucial determinants of grain yield in wheat (Triticum aestivum L.), while plant height (PH) independently affects lodging resistance, harvest index and final yield potential. Through comprehensive genetic analysis of a recombinant inbred line population derived from AS420 (large-spike line) × Lunxuan987 (LX987, modern cultivar), we identified 36 spike-specific QTLs (22 for SL and 14 for SN) with 22 showing environments stability, and 12 additional PH QTLs with 13 showing environmental stability, using 55 K SNP genotyping across six environments. A pleiotropic locus (QPh/Sl/Sn.cau-2D.1) on chromosome 2DS co-regulated PH, SL and SN (8.4-19.75% phenotypic variance (PVE)), and a 2,089-bp deletion on Ppd-D1 promoter was identified as the causal variant. In addition, we characterized REGULATOR OF GRAIN NUMBER1 (TaRGN-D1, TraesCS3D02G265100), encoding R2R3-MYB transcription factor, as the candidate gene for a major SN QTL QSn.cau-3D (6.5% PVE). Functional studies revealed a stronger transcriptional activation by the AS420-derived TaRGN-D1 promoter compared to LX987, correlating with elevated expression during spike development. Field validation confirmed the positive effect of TaRGN-D1 on SN without altering PH. Our findings not only identify new QTLs for wheat molecular breeding of high-yielding purpose, but also deepen our genetic comprehension on PH, SL, and SN regulation in this crop, thereby laying a valuable foundation for future wheat improvement strategies.

Indexed as

Plant ProteinsQuantitative Trait LociTriticumChromosome MappingChromosomes, PlantEdible GrainGene Expression Regulation, PlantGenotypePhenotypePlant BreedingPolymorphism, Single NucleotidePlant Proteins

Identifiers

PMID41123686

What OpenQuestion holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.