Evidence map›Paper›PMID 41122686›Full record

ArticleFrontiers in cellular and infection microbiology2025

Absolute quantification of VBNC state formation and resuscitation in alcohol-producing

Shuo Zhao, Chenpu Dou, Yang Yang, Jiazheng Wang, Meng Li, Bin Yang, Jing Yuan, Jian Zhang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Frontiers in cellular and infection microbiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Shuo Zhao *Department of Bacteriology, Capital Center for Children's Health, Capital Medical University, Capital Institute of Pediatrics, Beijing, China.
Chenpu Dou *Department of Bacteriology, Capital Center for Children's Health, Capital Medical University, Capital Institute of Pediatrics, Beijing, China.
Yang YangDepartment of Bacteriology, Capital Center for Children's Health, Capital Medical University, Capital Institute of Pediatrics, Beijing, China.
Jiazheng WangDepartment of Clinical Laboratory Medicine, The First Affiliated Hospital of Shandong First Medical University & Shandong Provincial Qianfoshan Hospital, Shandong Medicine and Health Key Laboratory of Laboratory Medicine, Jinan, Shandong, China.
Meng LiDepartment of Neurosurgery, North China University of Science and Technology Affiliated Hospital, Tangshan, China.
Bin YangDepartment of Neurology, North China University of Science and Technology Affiliated Hospital, Tangshan, China.
Jing YuanDepartment of Bacteriology, Capital Center for Children's Health, Capital Medical University, Capital Institute of Pediatrics, Beijing, China.
Jian ZhangDepartment of Neurosurgery, Capital Center for Children's Health, Capital Medical University, Capital Institute of Pediatrics, Beijing, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Introduction: Many bacterial species in the viable but non-culturable (VBNC) state pose a considerable risk to public health, primarily because of their ability to persist and resuscitate in the environment and their enhanced ability to escape detection. Our research revealed that high alcohol-producing Methods: For precise quantification, we optimized PMA concentration between 5 μM and 200 μM and adjusted the incubation time from 5 to 30 minutes. Subsequently, we established real-time quantitative PCR (qPCR) and droplet digital PCR (ddPCR) methods to count HiAlc Results: These methods produced methodological and technically comparable counts using KP, Discussion: This is a method for direct quantification that does not require an external standard curve reference. This method could be an innovative tool for quantifying viable cells of

Indexed as

EthanolKlebsiella pneumoniaeMicrobial ViabilityAnimalsAnti-Bacterial AgentsBacterial LoadCiprofloxacinFecesGastrointestinal MicrobiomeKlebsiella InfectionsMiceReal-Time Polymerase Chain ReactionAnti-Bacterial AgentsCiprofloxacinEthanolciprofloxacinddPCRHiAlc KpnresuscitationVBNC stateviable cell counting

Identifiers

PMID41122686
PMCPMC12535994

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.