ArticleFrontiers in public health2025
A socioenvironmental approach to the nosogenic potential of freshwaters with presence of thermotolerant free-living amoebae in Costa Rica.
Article in Frontiers in public health, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Introduction: Within the group of free-living amoebae (FLA), the genus Materials and methods: Water and sediment samples were collected from 24 locations. Parameters such as temperature, pH, electrical conductivity, and dissolved oxygen in water were measured. In sediment, analyses were performed for metals, cations, and texture. Water samples were also filtered, cultured, and subjected to molecular analyses to determine the presence of FLA. Additionally, a KAP survey was conducted among 72 individuals during the fieldwork. Results: FLA were isolated at 42 °C from 22 sites, 7 of which tested positive for Conclusion: This study represents a first monitoring effort to determine the frequency of FLA in surface waters of Costa Rica, indicating the presence of thermotolerant vahlkampfiid FLA in non-thermal freshwaters with the ability to proliferate at high temperatures. The KAP survey revealed a low level of knowledge and deficiencies in the management and prevention of PAM risk among the population exposed to natural freshwater reservoirs. These findings must drive health promotion, communication, and education strategies in the local population exposed to risky recreational activities.
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