Evidence map›Paper›PMID 41121173›Full record

ArticleVirology journal2025

Effect of monkeypox virus B14R protein fragments binding to IL-1β on apoptosis in HeLa cells.

Xiaojuan Zhu, Yunfeng Shan, Qiao Qiao, Fengjuan Shi, Kangchen Zhao, Jinlei Guo, Tao Wu, Shuo Ning, Heng Rong, Yuhan Ding and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Virology journal, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Xiaojuan ZhuNHC Key Laboratory of Enteric Pathogenic Microbiology, Jiangsu Provincial Medical Key Laboratory of Pathogenic Microbiology in Emerging Major Infectious Diseases, Jiangsu Provincial Center for Disease Control and Prevention, Nanjing, 210009, China.
Yunfeng ShanNHC Key Laboratory of Enteric Pathogenic Microbiology, Jiangsu Provincial Medical Key Laboratory of Pathogenic Microbiology in Emerging Major Infectious Diseases, Jiangsu Provincial Center for Disease Control and Prevention, Nanjing, 210009, China.
Qiao QiaoNHC Key Laboratory of Enteric Pathogenic Microbiology, Jiangsu Provincial Medical Key Laboratory of Pathogenic Microbiology in Emerging Major Infectious Diseases, Jiangsu Provincial Center for Disease Control and Prevention, Nanjing, 210009, China.
Fengjuan ShiNHC Key Laboratory of Enteric Pathogenic Microbiology, Jiangsu Provincial Medical Key Laboratory of Pathogenic Microbiology in Emerging Major Infectious Diseases, Jiangsu Provincial Center for Disease Control and Prevention, Nanjing, 210009, China.
Kangchen ZhaoNHC Key Laboratory of Enteric Pathogenic Microbiology, Jiangsu Provincial Medical Key Laboratory of Pathogenic Microbiology in Emerging Major Infectious Diseases, Jiangsu Provincial Center for Disease Control and Prevention, Nanjing, 210009, China.
Jinlei GuoNHC Key Laboratory of Enteric Pathogenic Microbiology, Jiangsu Provincial Medical Key Laboratory of Pathogenic Microbiology in Emerging Major Infectious Diseases, Jiangsu Provincial Center for Disease Control and Prevention, Nanjing, 210009, China.
Tao WuNHC Key Laboratory of Enteric Pathogenic Microbiology, Jiangsu Provincial Medical Key Laboratory of Pathogenic Microbiology in Emerging Major Infectious Diseases, Jiangsu Provincial Center for Disease Control and Prevention, Nanjing, 210009, China.
Shuo NingNHC Key Laboratory of Enteric Pathogenic Microbiology, Jiangsu Provincial Medical Key Laboratory of Pathogenic Microbiology in Emerging Major Infectious Diseases, Jiangsu Provincial Center for Disease Control and Prevention, Nanjing, 210009, China.
Heng RongNHC Key Laboratory of Enteric Pathogenic Microbiology, Jiangsu Provincial Medical Key Laboratory of Pathogenic Microbiology in Emerging Major Infectious Diseases, Jiangsu Provincial Center for Disease Control and Prevention, Nanjing, 210009, China.
Yuhan DingNHC Key Laboratory of Enteric Pathogenic Microbiology, Jiangsu Provincial Medical Key Laboratory of Pathogenic Microbiology in Emerging Major Infectious Diseases, Jiangsu Provincial Center for Disease Control and Prevention, Nanjing, 210009, China.
Yiyue GeNHC Key Laboratory of Enteric Pathogenic Microbiology, Jiangsu Provincial Medical Key Laboratory of Pathogenic Microbiology in Emerging Major Infectious Diseases, Jiangsu Provincial Center for Disease Control and Prevention, Nanjing, 210009, China.
Lunbiao CuiNHC Key Laboratory of Enteric Pathogenic Microbiology, Jiangsu Provincial Medical Key Laboratory of Pathogenic Microbiology in Emerging Major Infectious Diseases, Jiangsu Provincial Center for Disease Control and Prevention, Nanjing, 210009, China. lbcui@jscdc.cn.

Funding

National Key R&D Program of China 2023YFC2605100Natural Science Foundation of Jiangsu Province BK20231374Scientific Research Project of Jiangsu Commission of Health M2024024
6 · The paper itself

Abstract

backgroundInterleukin-1 beta (IL-1β) is a key mediator of antiviral immunity, and B14R proteins encoded by monkeypox virus (MPV) can interfere with its signaling. This study investigates the differences in IL-1β-binding capacity and apoptotic regulatory effects between two B14R protein isoforms encoded by MPV clades: the truncated B14R-180 (West African clade) and the full-length B14R-326 (Central African clade).

methodsThis study employed bioinformatics approaches to predict the binding affinity between B14R and IL-1β, and validated the findings through Enzyme-Linked Immunosorbent Assay (ELISA), flow cytometry, Quantitative RT-PCR (qRT-PCR), and Western blot analyses.

resultsthe research demonstrates that B14R-180 exhibits superior binding efficiency to IL-1β compared to B14R-326, attributed to more hydrogen bonds (9 vs. 7), lower binding free energy (ΔG = -12.4 vs. -2.0 kJ/mol), and optimized binding interfaces. ELISA confirms the significantly higher binding affinity of B14R-180 for IL-1β (P < 0.05), with stronger competitive inhibition by anti-IL-1β antibodies in vaccinia-immune sera. Flow cytometry reveals that the combination of B14R-180 and IL-1β significantly reduces cell apoptosis rates in HeLa cells after 48 h (h) (P < 0.05), whereas B14R-326 has no significant effect. Subsequent qRT-PCR indicates that the B14R-180/IL-1β co-treatment downregulates the transcriptional expression of caspase-3, caspase-8, caspase-9, and caspase-10 (P < 0.001). Notably, caspase-3 exhibited dual-level suppression, with significant reductions in both mRNA and protein abundance.

conclusionsThese results reveal distinct anti-apoptotic profiles of B14R isoforms and suggest that B14R-180 may enhance MPV immune evasion through high-affinity IL-1β binding. These findings provide critical mechanistic insights to guide the development of MPV vaccines and targeted therapeutic strategies.

Indexed as

ApoptosisInterleukin-1betaMonkeypox virusViral ProteinsComputational BiologyEnzyme-Linked Immunosorbent AssayFlow CytometryHeLa CellsHumansProtein BindingReal-Time Polymerase Chain ReactionInterleukin-1betaViral ProteinsApoptosisB14R-180 proteinB14R-326 proteinInterleukin-1β (IL-1β)Monkeypox virus (MPV)

Identifiers

PMID41121173
PMCPMC12538970

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.