Evidence map›Paper›PMID 41120853›Full record

ArticleBMC microbiology2025

Genotyping of Coxiella burnetii isolates in Iran using Multiple-locus variable-number tandem repeat analysis (MLVA).

Fatemeh Dolatkhah, Safoura Moradkasani, Ashraf Mohabati Mobarez, Mohammad Khalili, Ehsan Mostafavi, Kayvan Tadayon, Saber Esmaeili

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Article in BMC microbiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

7 authors.

Fatemeh Dolatkhah *Department of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
Safoura Moradkasani *WHO Collaborating Centre for Vector-Borne Diseases, Department of Epidemiology and Biostatistics, Research Centre for Emerging and Reemerging Infectious Diseases, Pasteur Institute of Iran, Tehran, Iran.
Ashraf Mohabati Mobarez *Department of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
Mohammad KhaliliDepartment of Pathobiology, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran.
Ehsan MostafaviWHO Collaborating Centre for Vector-Borne Diseases, Department of Epidemiology and Biostatistics, Research Centre for Emerging and Reemerging Infectious Diseases, Pasteur Institute of Iran, Tehran, Iran.
Kayvan TadayonBovine Tuberculosis Reference Laboratory, Razi Vaccine & Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran.
Saber EsmaeiliWHO Collaborating Centre for Vector-Borne Diseases, Department of Epidemiology and Biostatistics, Research Centre for Emerging and Reemerging Infectious Diseases, Pasteur Institute of Iran, Tehran, Iran. dr.saberesmaeili@gmail.com.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

introductionCoxiella burnetii is the etiological agent of Q fever, a zoonotic infection recognized in various regions of Iran. Despite its significance, few studies have focused on determining C. burnetii genotypes in Iran. This study aimed to assess the genetic diversity of C. burnetii in Iran using the Multiple-Locus Variable-Number Tandem Repeat Analysis (MLVA) method.

methodsA total of 26 C. burnetii-positive specimens were selected from a repository of 119 specimens collected from various locations across Iran. These samples were previously identified as C. burnetii using nested PCR and TaqMan real-time PCR techniques. In this study, the MLVA method was utilized for genotyping C. burnetii isolates.

resultsGenotyping results from the 26 C. burnetii-positive samples indicated that nearly all loci were informative for genotyping, except two loci (ms23 and ms26). Cluster analysis of the MLVA profiles identified 13 clusters, along with 40 singleton genotypes. Iranian isolates were distributed across a single cluster (Cluster 13), which consisted exclusively of Iranian strains, and 24 unique singleton genotypes, highlighting the genetic diversity of C. burnetii circulating within the region. DISCUSSION AND

conclusionThe genotypic characteristics of C. burnetii observed in Iran differed significantly from those found in other global regions, suggesting unique local adaptations of this microorganism. To enhance our understanding of the epidemiology of Q fever in Iran, further large-scale studies are required to evaluate the distribution of C. burnetii genotypes across various geographic areas, host species, and environmental reservoirs.

Indexed as

Coxiella burnetiiMinisatellite RepeatsQ FeverAnimalsCluster AnalysisDNA, BacterialGenetic VariationGenotypeGenotyping TechniquesHumansIranPhylogenyDNA, BacterialCoxiella burnetiiGenotypingIranMLVAMolecular epidemiologyQ fever

Identifiers

PMID41120853
PMCPMC12539059

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