Evidence map›Paper›PMID 41120712›Full record

ArticleScientific reports2025

Distinct roles of amniotic membrane epithelial (hAEC) and mesenchymal stromal cells (hAMSC) in amniotic membrane-driven wound healing.

M Alcaraz, I Hernández-Mármol, J M Puente-Cuadrado, M Rodríguez-Valiente, A R Silini, O Parolini, F J Nicolás

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

M Alcaraz *Soluciones de biología molecular y celular en medicina regenerativa, Health Sciences PhD Program, Universidad Católica de Murcia (UCAM), Campus de los Jerónimos no 135, Guadalupe, Murcia, 30107, Spain.
I Hernández-Mármol *Regeneración Oncología Molecular y TGF-β, Trasplantehematopoyético/terapia celular, Instituto Murciano de Investigación Biosanitaria Pascual Parrilla-IMIB, Murcia, 30120, Spain.
J M Puente-Cuadrado *Regeneración Oncología Molecular y TGF-β, Trasplantehematopoyético/terapia celular, Instituto Murciano de Investigación Biosanitaria Pascual Parrilla-IMIB, Murcia, 30120, Spain.
M Rodríguez-ValienteSoluciones de biología molecular y celular en medicina regenerativa, Health Sciences PhD Program, Universidad Católica de Murcia (UCAM), Campus de los Jerónimos no 135, Guadalupe, Murcia, 30107, Spain.
A R SiliniCentro di Ricerca E. Menni, Fondazione Poliambulanza Istituto Ospedaliero, Brescia, 25124, Italy.
O ParoliniDepartment of Life Science and Public Health, Università Cattolica del Sacro Cuore, Rome, 00168, Italy.
F J NicolásRegeneración Oncología Molecular y TGF-β, Trasplantehematopoyético/terapia celular, Instituto Murciano de Investigación Biosanitaria Pascual Parrilla-IMIB, Murcia, 30120, Spain. franciscoj.nicolas2@carm.es.

Funding

Instituto de Salud Carlos III PI2101339
6 · The paper itself

Abstract

Human skin wound healing is a complex process involving sequential cellular events to restore skin integrity. Chronic wounds fail to heal due to impaired keratinocyte activation and dysregulated cytokine signaling. The human amniotic membrane (hAM), a perinatal derivative, is widely used in hospitals due to its therapeutic effects on wound healing. hAM contains two main cell types: human Amniotic Epithelial Cells (hAEC) and human Amniotic Mesenchymal Stromal Cells (hAMSC). This study isolated and cultured both cell types, using their conditioned media in assays for cell migration, proliferation, and TGF-β cell-cycle arrest rescue. While hAEC-conditioned media only had similar effects to hAM in cell migration, hAMSC-conditioned media demonstrated superior efficacy in this and other assays. These findings suggest that hAMSC are the primary contributors to AM's beneficial effects in chronic wound healing, highlighting their potential for targeted therapeutic applications.

Indexed as

AmnionEpithelial CellsMesenchymal Stem CellsWound HealingCell MovementCell ProliferationCells, CulturedCulture Media, ConditionedHumansTransforming Growth Factor betaCulture Media, ConditionedTransforming Growth Factor betaAmniotic membraneChronic woundsConditioned mediahAEChAMSCTGF-βWound healing

Identifiers

PMID41120712
PMCPMC12540749

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.