Evidence map›Paper›PMID 41117625›Full record

ArticleJournal of clinical microbiology2025

Rapid detection of gram-negative antimicrobial resistance determinants directly from positive blood culture broths using a multiplex PCR system.

Stefanie Marxreiter, Jamie Marino, Katrina Callan, Judith Hargrave, Tricia Alston, Kathy Fauntleroy, Amy Robertson, Barry N Kreiswirth, Liang Chen, Mariana Castanheira and 5 more

Abstract readEvaluation Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of clinical microbiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Global epidemiology of carbapenem-resistantJAC-antimicrobial resistance · 2026
    Review
  2. Occurrence of Phenotypic Multidrug-ResistantAntibiotics (Basel, Switzerland) · 2026
    Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Stefanie MarxreiterbioMérieux, Inc., Salt Lake City, Utah, USA.
Jamie MarinoDepartment of Pathology and Laboratory Medicine, Weill Cornell Medicine, New York, New York, USA.
Katrina CallanNewYork-Presbyterian Hospital, Weill Cornell Medical Center, New York, New York, USA.
Judith HargraveNewYork-Presbyterian Hospital, Weill Cornell Medical Center, New York, New York, USA.
Tricia AlstonNewYork-Presbyterian Hospital, Weill Cornell Medical Center, New York, New York, USA.
Kathy FauntleroyNewYork-Presbyterian Hospital, Weill Cornell Medical Center, New York, New York, USA.
Amy RobertsonNewYork-Presbyterian Hospital, Weill Cornell Medical Center, New York, New York, USA.
Barry N KreiswirthCenter for Discovery and Innovation, Hackensack Meridian Health, Nutley, New Jersey, USA.
Liang ChenSchool of Pharmacy and Pharmaceutical Sciences, University of Buffalo, Buffalo, New York, USA.ORCID 0000-0001-5845-2235
Mariana CastanheiraElement Materials Technology (JMI Laboratories), North Liberty, Iowa, USA.
Matthew HockinbioMérieux, Inc., Salt Lake City, Utah, USA.
Andrew C HemmertbioMérieux, Inc., Salt Lake City, Utah, USA.
Amy DavisbioMérieux, Inc., Salt Lake City, Utah, USA.
Michael J SatlinDepartment of Pathology and Laboratory Medicine, Weill Cornell Medicine, New York, New York, USA.ORCID 0000-0003-4711-5334
Lars F WestbladeDepartment of Pathology and Laboratory Medicine, Weill Cornell Medicine, New York, New York, USA.ORCID 0000-0003-3079-7674

Funding

bioMérieux SA RCA 033-02-01
6 · The paper itself

Abstract

Currently available rapid blood culture diagnostics detect few gram-negative resistance determinants, limiting their clinical utility. We prospectively evaluated the prototype BIOFIRE FILMARRAY Antimicrobial Resistance (AMR) Panel, a rapid multiplex PCR test that detects 31 AMR genes, on residual positive blood culture broths from patients with gram-negative bacteremia due to five target organisms at a New York City hospital. Predicted antimicrobial resistance based on the AMR Panel was compared to results from broth microdilution testing of bloodstream isolates recovered in culture. A simulated stewardship study assessed opportunities for the optimization of therapy if the AMR Panel results had been available for patient care in real time. We enrolled 148 patients with gram-negative bacteremia (

Indexed as

BacteremiaDrug Resistance, BacterialGram-Negative BacteriaGram-Negative Bacterial InfectionsMultiplex Polymerase Chain ReactionAnti-Bacterial AgentsBlood CultureFemaleHumansMaleMicrobial Sensitivity TestsNew York CityProspective StudiesSensitivity and SpecificityAnti-Bacterial Agentsantimicrobial resistanceblood culturesgram-negative bacteriamultiplex PCR

Identifiers

PMID41117625
PMCPMC12607888

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.