Evidence map›Paper›PMID 41117399›Full record

ArticleLiver international : official journal of the International Association for the Study of the Liver2025

Navigating the Hepatic Immune Landscape With Fine Needle Aspiration of the Liver-An Emerging Technique.

Kate Diana Lynch, Fabiola Curion, Hing-Yuen Yeung, Charlotte Rich-Griffin, Devika Agarwal, Helen Ferry, Andrew Slater, Emma Louise Culver, Roger William Chapman, Satish Keshav and 2 more

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Article in Liver international : official journal of the International Association for the Study of the Liver, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Kate Diana LynchTranslational Gastroenterology and Liver Unit, NDM Experimental Medicine, University of Oxford, Oxford, UK.ORCID 0000-0001-7748-2165
Fabiola CurionNuffield Department of Medicine, Wellcome Centre for Human Genetics, University of Oxford, Oxford, UK.
Hing-Yuen YeungNuffield Department of Medicine, Wellcome Centre for Human Genetics, University of Oxford, Oxford, UK.
Charlotte Rich-GriffinNuffield Department of Medicine, Wellcome Centre for Human Genetics, University of Oxford, Oxford, UK.
Devika AgarwalNuffield Department of Medicine, Wellcome Centre for Human Genetics, University of Oxford, Oxford, UK.
Helen FerryTranslational Gastroenterology and Liver Unit, NDM Experimental Medicine, University of Oxford, Oxford, UK.
Andrew SlaterDepartment of Radiology, John Radcliffe Hospital, Oxford University, Hospitals NHS Foundation Trust, Oxford, UK.
Emma Louise CulverTranslational Gastroenterology and Liver Unit, NDM Experimental Medicine, University of Oxford, Oxford, UK.
Roger William ChapmanTranslational Gastroenterology and Liver Unit, NDM Experimental Medicine, University of Oxford, Oxford, UK.
Satish KeshavTranslational Gastroenterology and Liver Unit, NDM Experimental Medicine, University of Oxford, Oxford, UK.
Paul KlenermanTranslational Gastroenterology and Liver Unit, NDM Experimental Medicine, University of Oxford, Oxford, UK.
Calliope Athena DendrouNuffield Department of Medicine, Wellcome Centre for Human Genetics, University of Oxford, Oxford, UK.

Funding

PSC Support 260917KWWellcome Trust 200154/Z/15/ZWellcome Trust 204290/Z/16/ZWellcome Trust 211276/B/18/ZWellcome Trust 222426/Z/21/Z
6 · The paper itself

Abstract

BACKGROUND AND

aimsHepatic immune cell analysis is critical for understanding chronic inflammatory liver diseases, such as primary sclerosing cholangitis (PSC) and steatotic liver disease. However, liver immunoprofiling is limited due to reliance on end-stage disease liver explants. Fine needle aspiration (FNA) is a minimally invasive technique that can overcome these limitations. We evaluate the safety and efficacy of liver FNA to profile hepatic immune subsets in non-infectious liver conditions.

methodsFlow cytometry and single-cell RNA sequencing (scRNA-seq) were used to compare the hepatic immune cell composition and gene expression to that of matched peripheral blood mononuclear cells (PBMCs).

resultsWe obtained liver FNAs from 38 patients. The median pain score was 0. No serious adverse effects were reported. Flow cytometry demonstrated enrichment of CD69

conclusionsFNA sampling is a safe approach for investigating the inflammatory landscape of PSC and other liver diseases. Single-cell profiling reveals that FNAs capture tissue-specific immune cell types and gene expression differences, suggesting this sampling method may provide a basis for future experimental medicine analyses.

Indexed as

Cholangitis, SclerosingLiverAdultAgedBiopsy, Fine-NeedleFemaleFlow CytometryHumansKiller Cells, NaturalLeukocytes, MononuclearMaleMiddle AgedRNA-SeqSingle-Cell Analysisfine needle aspirationflow cytometryliverprimary sclerosing cholangitissingle‐cellsteatohepatitis

Identifiers

PMID41117399
PMCPMC12539073

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.