Evidence map›Paper›PMID 41116609›Full record

ArticleProteomics2025

Methionine Oxidation Footprinting in Intact Proteins (MOFIP) Using Top-Down Proteomics.

Anju Teresa Sunny, Kellye A Cupp-Sutton, Zhitao Zhao, Trishika Chowdhury, Yanting Guo, Si Wu

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Proteomics, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Anju Teresa SunnyDepartment of Chemistry and Biochemistry, University of Alabama, Tuscaloosa, Alabama, USA.
Kellye A Cupp-SuttonDepartment of Chemistry and Biochemistry, University of Alabama, Tuscaloosa, Alabama, USA.
Zhitao ZhaoDepartment of Chemistry and Biochemistry, University of Oklahoma, Norman, Oklahoma, USA.
Trishika ChowdhuryDepartment of Chemistry and Biochemistry, University of Alabama, Tuscaloosa, Alabama, USA.
Yanting GuoDepartment of Chemistry and Biochemistry, University of Oklahoma, Norman, Oklahoma, USA.
Si WuDepartment of Chemistry and Biochemistry, University of Alabama, Tuscaloosa, Alabama, USA.ORCID 0000-0002-6346-7359

Funding

Translation of immunologic technologies from basic research into pre-clinical nonU19AI062629 · NIAID · OKLAHOMA MEDICAL RESEARCH FOUNDATION · PI COGGESHALL, KENNETH MARK · 2004 to 2023
$56.2M
Structure-Function Studies of MsvR, a Methanogen-Specific Transcriptional RegulaP20GM103640 · NIGMS · UNIVERSITY OF OKLAHOMA · PI THOMAS, LEONARD M · 2012 to 2021
$20.4M
Protein Production and Characterization CoreP30GM145423 · NIGMS · UNIVERSITY OF OKLAHOMA · PI ANN H WEST · 2022 to 2026
$6.9M
Quantitative Analysis of Serum Autoantibody Repertories in Systemic Lupus ErythematosusR01AI141625 · NIAID · UNIVERSITY OF OKLAHOMA · PI SINGH, SHANTERI, SMITH, KENNETH MICHAEL · 2019 to 2023
$2.1M
IMAT-ITCR Collaboration: Integrated Computational Framework for Validation and Quantification of Intact Proteoforms in Patient-Derived Ovarian Cancer SpheroidsR61CA297964 · NCI · UNIVERSITY OF ALABAMA IN TUSCALOOSA · PI Anthony W.G. Burgett, Si Wu · 2025 to 2026
$773k
Institutional Development Awards (IDeA)National Institute of Allergy and Infectious Diseases 2U19AI062629National Institute of Allergy and Infectious Diseases R01AI141625National Institute of General Medical Sciences of the National Institutes of Health P20GM103640National Institute of General Medical Sciences of the National Institutes of Health P30GM145423NCI NIH HHS R61 CA297964NIAID NIH HHS R01 AI141625NIAID NIH HHS U19 AI062629NIGMS NIH HHS P20 GM103640NIGMS NIH HHS P30 GM145423NIH NIAID NIH/NIAID2U19AI062629NIH NIAID R01AI141625OCAST HR23-169Protein Production and Characterization Core (PPC) facilityUniversity of Alabama's startup fund
6 · The paper itself

Abstract

Mass spectrometry (MS)-based proteomics methods, including protein footprinting methods such as hydrogen-deuterium exchange mass spectrometry (HDX-MS) and hydroxyl radical footprinting (HRF), can give unique insight into protein structure and interactions. These methods primarily utilize bottom-up proteomics techniques that require the digestion of intact proteins into small peptides before MS analysis. This digestion can obscure structural information relevant to the function of the intact proteoforms. Here, we have developed a novel top-down footprinting method, Methionine Oxidation Footprinting in Intact Proteins (MOFIP), to probe solvent accessibility in intact proteoforms. For MOFIP, natively folded protein lysates are incubated with and without hydrogen peroxide (H

Indexed as

Escherichia coli ProteinsMethionineProtein FootprintingProteomicsEscherichia coliHydrogen PeroxideOxidation-ReductionEscherichia coli ProteinsHydrogen PeroxideMethionine

Identifiers

PMID41116609
PMCPMC12788183

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.