Evidence map›Paper›PMID 41115906›Full record

ArticleScientific reports2025

Colistin- and cefotaxime-resistant Shiga toxin-producing Escherichia coli (STEC) in buffalo meat.

Khalid Ibrahim Sallam, Mohamed Z Sayed-Ahmed, Hanan Ahmed Zaher, Amira ElSayeh, Hilal A Thaibah, Moaddey Alfarhan, Sarfaraz Ahmad, Nawazish Alam, Samir Mohammed Abd-Elghany

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Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

9 authors.

Khalid Ibrahim Sallam *Food Hygiene, Safety and Technology Department, Faculty of Veterinary Medicine, Mansoura University, Mansoura, 35516, Egypt. khalidsallam@mans.edu.eg.
Mohamed Z Sayed-Ahmed *Department of Clinical Practice, College of Pharmacy, Jazan University, Jazan, 45142, Saudi Arabia. mzakaria@jazanu.edu.sa.
Hanan Ahmed ZaherFood Hygiene, Safety and Technology Department, Faculty of Veterinary Medicine, Mansoura University, Mansoura, 35516, Egypt.
Amira ElSayehFood Hygiene, Safety and Technology Department, Faculty of Veterinary Medicine, Mansoura University, Mansoura, 35516, Egypt.
Hilal A ThaibahDepartment of Clinical Practice, College of Pharmacy, Jazan University, Jazan, 45142, Saudi Arabia.
Moaddey AlfarhanDepartment of Clinical Practice, College of Pharmacy, Jazan University, Jazan, 45142, Saudi Arabia.
Sarfaraz AhmadDepartment of Clinical Practice, College of Pharmacy, Jazan University, Jazan, 45142, Saudi Arabia.
Nawazish AlamDepartment of Clinical Practice, College of Pharmacy, Jazan University, Jazan, 45142, Saudi Arabia.
Samir Mohammed Abd-ElghanyFood Hygiene, Safety and Technology Department, Faculty of Veterinary Medicine, Mansoura University, Mansoura, 35516, Egypt. drsamir@mans.edu.eg.

Funding

The authors gratefully acknowledge the funding of the Deanship of Graduate Studies and Scientific Research, Jazan University, Saudi Arabia, through Project number: (JU- 20250272 -DGSSR- RP -2025). JU- 20250272 -DGSSR- RP -2025the Deanship of Graduate Studies and Scientific Research, Jazan University, Saudi Arabia, through Project number: (JU- 20250272 -DGSSR- RP -2025). JU- 20250272 -DGSSR- RP -2025
6 · The paper itself

Abstract

In this study, Shiga toxin-producing E. coli (STEC) were detected in 64% of all tested buffalo meat samples, distributed as 62% of buffalo meat chops and 66% of ground buffalo meat. Six serotypes (O55:H7, O111:H8, O145, O157:H7, O26:H11, and O103:H2) were identified, of which, O55:H7 (37.26%) and O111:H8 (21.29%) were the most predominant. The eaeA, stx1, stx2, and EHEC-hlyA were detected by multiplex PCR in all E. coli isolates at different percentages, and multiple virulence patterns with stx2/stx1/eae were the most prevalent pattern indicating that the serotypes will be more virulent in pathogenesis. The isolates showed high resistance rates against Erythromycin (100%), Clindamycin (86.7%), Nalidixic acid (84.4%), Cefepime (73.4%), and Ampicillin (62.7%), Cefazolin (53.2%) and Tetracycline (51.7%) and most of the isolates (73%) were classified as multi-drug resistant (MDR). For the first time, we identified a high frequency of Cefotaxime-resistant (28.5%) and Colistin-resistant (16.3%) E. coli from buffalo meat and this becomes a huge problem because Cefotaxime and colistin are typically used as a last resort to treat complex infections caused by multidrug-resistant Gram-negative bacilli. The emergence of virulent and multidrug-resistant E. coli strains in food seriously threatens human health. This highlights the need for antimicrobial stewardship programs in developing countries like Egypt to minimize the spread and emergence of these strains and prevent their dissemination to humans.

Indexed as

Anti-Bacterial AgentsCefotaximeColistinDrug Resistance, BacterialDrug Resistance, Multiple, BacterialMeatShiga-Toxigenic Escherichia coliAnimalsBuffaloesEscherichia coli ProteinsFood MicrobiologyMicrobial Sensitivity TestsSerogroupAnti-Bacterial AgentsCefotaximeColistinEscherichia coli ProteinsAntimicrobial resistanceEscherichia coliMulti-drug resistantShiga toxinSTECVirulence genes

Identifiers

PMID41115906
PMCPMC12537903

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.