Evidence map›Paper›PMID 41115376›Full record

ArticleBioelectrochemistry (Amsterdam, Netherlands)2026

Resolving nanosecond kinetics of the optical membrane potential in pulsed electric fields.

Iurii Semenov, Giedre Silkuniene, Mantas Silkunas, Olga N Pakhomova, Joel N Bixler, Bennett L Ibey, Mark A Keppler, Andrei G Pakhomov

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Bioelectrochemistry (Amsterdam, Netherlands), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Iurii SemenovFrank Reidy Research Center for Bioelectrics, Old Dominion University, Norfolk, VA 23508, USA.
Giedre SilkunieneFrank Reidy Research Center for Bioelectrics, Old Dominion University, Norfolk, VA 23508, USA.
Mantas SilkunasFrank Reidy Research Center for Bioelectrics, Old Dominion University, Norfolk, VA 23508, USA.
Olga N PakhomovaFrank Reidy Research Center for Bioelectrics, Old Dominion University, Norfolk, VA 23508, USA.
Joel N BixlerAir Force Office of Scientific Research, 875 North Randolph Street, Arlington, VA 22203, USA.
Bennett L IbeyAir Force Office of Scientific Research, 875 North Randolph Street, Arlington, VA 22203, USA.
Mark A KepplerTexas A&M University, Department of Biomedical Engineering, 101 Bizzell Street, College Station, TX 77843, USA.
Andrei G PakhomovFrank Reidy Research Center for Bioelectrics, Old Dominion University, Norfolk, VA 23508, USA. Electronic address: 2andrei@pakhomov.net.

Funding

Targeted Neuromodulation by Nanosecond Pulsed Electric FieldsR21EY034258 · NEI · OLD DOMINION UNIVERSITY · PI PAKHOMOV, ANDREI G · 2022 to 2024
$640k
Next Generation Temporal Interference Stimulation for Non-Invasive NeuromodulationR21EY034803 · NEI · OLD DOMINION UNIVERSITY · PI PAKHOMOV, ANDREI G · 2023 to 2025
$640k
NEI NIH HHS R21 EY034258NEI NIH HHS R21 EY034803
6 · The paper itself

Abstract

The primary bioeffect of pulsed electric fields (PEFs) is cell membrane charging, which determines a plethora of downstream events. While the kinetics of de- and hyperpolarization at the cathode- and anode-facing cell hemispheres should be the same, studies using pulsed laser strobe microscopy have reported asymmetrical charging in mammalian cells exposed to brief PEFs. To validate these anomalies, we measured charging kinetics with FluoVolt and Di-8-ANEPPS potentiometric dyes in spheroidal CHO cells exposed to 1-μs PEFs at 0-350 V/cm. Both dyes exhibited emission changes dependent on the electric field incidence angle, symmetrically at cathodic and anodic hemispheres. The changes scaled linearly with electric field strength up to at least 350 V/cm for Di-8-ANEPPS, but only to ∼200 V/cm for FluoVolt. Time constants measured from the optical membrane potential kinetics did not differ between the dyes, between cathodic and anodic cell poles, or between membrane charging and relaxation. The time constants followed the theoretical dependence on cell radius for 1.6 μF/cm

Indexed as

Cell MembraneElectricityMembrane PotentialsAnimalsCHO CellsCricetinaeCricetulusKineticsPyridinium CompoundsTime Factors1-(3-sulfonatopropyl)-4-(beta-(2-(di-n-octylamino)-6-naphthyl)vinyl)pyridinium betainePyridinium CompoundsElectropermeabilizationElectroporationElectrostimulationMembrane chargingMembrane impedanceNanosecond pulsesTime constant

Identifiers

PMID41115376
PMCPMC12553484

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.