ArticleProceedings of SPIE--the International Society for Optical Engineering2025
A Dual-Camera High-Resolution Hyperspectral Imaging System for the Retina.
Article in Proceedings of SPIE--the International Society for Optical Engineering, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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7 authors.
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Abstract
Hyperspectral imaging (HSI) emerged as a powerful tool for biomedical applications, particularly in the analysis and discrimination of biological tissues. In this study, we developed a dual-camera system that integrates hyperspectral imaging with high-resolution RGB imaging to capture detailed retinal images. We tested different pan-sharpening algorithms to enhance the spatial resolution of the hyperspectral images, combining the spectral details of HSI with the spatial details of RGB imaging. We developed algorithms to estimate the diameter of retinal vessels and the oxygenation rate. We validated our algorithms by imaging a mouse retinal phantom and then by imaging mice under anesthesia. Our systems showed the ability to resolve fine structural details, including small blood vessels. We found that out of the tested methods, PSGAN offered the best pansharpened image both quantitatively and qualitatively, with a root-mean-squared error (RMSE) score of 2.15 × 10
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