ArticleInternational journal of clinical pediatric dentistry2025
Evaluation of Cytotoxicity of Premixed Triple Antibiotic Paste on Stem Cells of Apical Papilla Using MTT Assay: An
Article in International journal of clinical pediatric dentistry, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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Who cites it
1 citing paper in PubMed.
- Direct effect of nitrofurantoin on dental pulp stem cell viability and odontoblastic differentiation potential.Journal of conservative dentistry and endodontics · 2026Article
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2 authors.
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Abstract
Background: Endodontic regenerative procedures are biologically based and intend to replace damaged dental tissues. This includes the restoration of the dentin-pulp complex through the use of stem cells. Root canal medications, particularly antibiotics, have shown significance in obtaining excellent therapeutic outcomes. However, high concentrations of some materials, like calcium hydroxide [Ca(OH) Aim: To evaluate the cytotoxic effects of premixed triple antibiotic paste (TAP) on stem cells of apical papilla (SCAPs) using methyl thiazolyl tetrazolium (MTT) assay. Materials and methods: Stem cells of apical papilla were isolated from the dental pulp tissue of extracted premolars and characterized using flow cytometry. The isolated cells were divided into four groups: Groups I, II, and III were treated with TAP at concentrations of 10, 25, and 50 μg/mL, respectively, while group IV served as the untreated control. The MTT assay was performed according to the manufacturer's protocol to assess cell viability. Evaluations were carried out at 1, 3, and 7 days following exposure to the TAP concentrations. Results: Cell viability was highest in groups treated with 10 and 25 μg/mL TAP, indicating minimal cytotoxicity across all evaluated time points-1, 3, and 7 days. In contrast, the group exposed to 50 μg/mL TAP demonstrated significantly reduced cell viability, indicating a dose-dependent increase in cytotoxicity. Conclusion: This study concludes that TAP at concentrations of 10 and 25 μg/mL demonstrates higher cell viability and lower cytotoxicity on SCAPs across 1, 3, and 7 days of evaluation. In contrast, a concentration of 50 μg/mL exhibited the most pronounced cytotoxic effects. While these findings suggest that lower concentrations of TAP may be safer for regenerative applications, further How to cite this article: Saravanan E, Ravindran V. Evaluation of Cytotoxicity of Premixed Triple Antibiotic Paste on Stem Cells of Apical Papilla Using MTT Assay: An
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