Evidence map›Paper›PMID 41113660›Full record

ArticlePain reports2025

Biotinidase as a novel biomarker for pain assessment in dairy cattle.

Miri Cohen-Zinder, Ariel Shabtay, Einav Shor-Shimoni, Hen H Honig, Aviv Asher, Shmuel Friedman, Maya Ross, Noa Yitzhak, Yael Shilo-Benjamini, Yael Salzer

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Article in Pain reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Miri Cohen-ZinderSustainable Ruminant Production Laboratory, Institute of Animal Science, Newe Ya'ar Research Center, Agricultural Research Organization-Volcani Institute, Ramat Yishay, Israel.
Ariel ShabtaySustainable Ruminant Production Laboratory, Institute of Animal Science, Newe Ya'ar Research Center, Agricultural Research Organization-Volcani Institute, Ramat Yishay, Israel.ORCID https://orcid.org/0000-0002-0672-7118
Einav Shor-ShimoniSustainable Ruminant Production Laboratory, Institute of Animal Science, Newe Ya'ar Research Center, Agricultural Research Organization-Volcani Institute, Ramat Yishay, Israel.
Hen H HonigInstitute of Animal Science, Agricultural Research Organization-Volcani Center, Rishon-Le'Zion, Israel.
Aviv AsherNorthern Agriculture R&D, MIGAL-Galilee Research Institute, Kiryat Shmona, Israel.
Shmuel FriedmanIsrael Dairy Board-National Service for Udder Health and Milk Quality.
Maya RossInstitute of Animal Science, Agricultural Research Organization-Volcani Center, Rishon-Le'Zion, Israel.
Noa YitzhakHuman and Animal Factors Engineering Laboratory, Institute of Agricultural and Biosystem Engineering, Agricultural Research Organization-Volcani Institute, Rishon-Le'Zion, Israel.
Yael Shilo-BenjaminiKoret School of Veterinary Medicine, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, Israel.
Yael SalzerHuman and Animal Factors Engineering Laboratory, Institute of Agricultural and Biosystem Engineering, Agricultural Research Organization-Volcani Institute, Rishon-Le'Zion, Israel.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Introduction: Freedom from pain is a key component of animal welfare. However, unlike in humans, objective methods for assessing and managing pain in farm animals remain limited. Pain assessment is particularly challenging in cattle, due to their inherently stoic nature. Objective: We investigated biotinidase, an enzyme with amino-exopeptidase activity on opioid neuropeptides, as a novel, short-term pain-biomarker in dairy cattle, in comparison with cortisol and substance P. Methods: Plasma levels of biotinidase, substance P, and cortisol were quantified by ELISA in Holstein cows subjected to 3 distinct pain stimuli: freeze branding (routine husbandry), topical application of capsaicin cream (nonclinical pain model), and spontaneous clinical mastitis. Results: Biotinidase levels increased significantly within 30 minutes after freeze branding compared to controls (1.36 difference of normalized values; Conclusion: These findings provide compelling new evidence that plasma biotinidase responds robustly to diverse pain stimuli in nonhuman mammals. Further studies should investigate its diagnostic performance individually, and alongside other biochemical, physiological, and behavioral indicators, to enhance pain assessment accuracy and of noninvasive pain detection protocols for farm animals.

Indexed as

Animal welfareBiotinidaseCapsaicinCortisolDairy cattleFreeze brandingMastitisPainSubstance P

Identifiers

PMID41113660
PMCPMC12528599

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.