ArticleFrontiers in cell and developmental biology2025
Functional characterization and phenotyping of RAB2A and Lactadherin/MFGE8 as boar sperm zona pellucida binding proteins.
Article in Frontiers in cell and developmental biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
1 citing paper in PubMed.
- Medium Composition Determines the Dynamics of Boar In Vitro Sperm Capacitation-Associated Events.International journal of molecular sciences · 2026Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
17 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mammalian fertilization begins with the species-specific binding of spermatozoa to the oocyte's zona pellucida (ZP), a process mediated by multiple surface proteins forming a functional receptor complex. Among them, the Ras oncogene family protein RAB2A and lactadherin/MFGE8 (p47/SED1) were previously identified as ZP-binding candidates in pig; however, their functional roles in sperm-oocyte interactions remained unconfirmed. This study aimed to evaluate their involvement in sperm-ZP binding by using antibody-blocking and competitive binding assays with porcine oocytes. In parallel, we validated the specificity and functional relevance of two in-house raised monoclonal antibodies, 5C5 and 1H9, targeting RAB2A and lactadherin/MFGE8, respectively, and further characterized these proteins in boar spermatozoa. Immunofluorescence detection indicated that both RAB2A and lactadherin/MFGE8 became accessible on the sperm surface upon capacitation. Their surface localization at this stage supports their potential involvement in the primary sperm-ZP interactions preceding acrosomal exocytosis. Moreover, the sperm-specific 5C5 antibody detected reduced RAB2A levels in ejaculates from men with abnormal sperm parameters. This highlights the potential of RAB2A as a biomarker of sperm quality and acrosomal integrity, with promising translational relevance from animal models to humans.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.