Evidence map›Paper›PMID 41112867›Full record

ArticleACS nanoscience Au2025

Delivery of Biomolecules into Individual Cells and Subcellular Compartments by Localized Electroporation via Nanopipette.

Fabio Marcuccio, Philip S Goff, Devkee M Vadukul, Fawaz Raja, Yilin Li, Ren Ren, Debjani Saha, Luca Magnani, Francesco A Aprile, Uma Anand and 6 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in ACS nanoscience Au, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. A Correlative SICM-OPM Platform for Surface and Volumetric Imaging in Live Cells.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors.

Fabio MarcuccioFaculty of Medicine, Imperial College London, Hammersmith Campus, Du Cane Road, London W12 0NN, U.K.ORCID https://orcid.org/0000-0003-4816-2896
Philip S GoffSchool of Health and Medical Sciences, City St George's, University of London, Cranmer Terrace, London SW17 0RE, U.K.
Devkee M VadukulDepartment of Chemistry, Imperial College London, Molecular Sciences Research Hub, White City Campus, 82 Wood Lane, London W12 0BZ, U.K.ORCID https://orcid.org/0000-0003-2073-0089
Fawaz RajaDepartment of Chemistry, Imperial College London, Molecular Sciences Research Hub, White City Campus, 82 Wood Lane, London W12 0BZ, U.K.
Yilin LiDepartment of Chemistry, Imperial College London, Molecular Sciences Research Hub, White City Campus, 82 Wood Lane, London W12 0BZ, U.K.
Ren RenFaculty of Medicine, Imperial College London, Hammersmith Campus, Du Cane Road, London W12 0NN, U.K.ORCID https://orcid.org/0000-0001-8288-8012
Debjani SahaThe Breast Cancer Now Toby Robins Research Centre, The Institute of Cancer Research, 123 Old Brompton Road, London SW7 3RP, U.K.
Luca MagnaniThe Breast Cancer Now Toby Robins Research Centre, The Institute of Cancer Research, 123 Old Brompton Road, London SW7 3RP, U.K.
Francesco A AprileDepartment of Chemistry, Imperial College London, Molecular Sciences Research Hub, White City Campus, 82 Wood Lane, London W12 0BZ, U.K.ORCID https://orcid.org/0000-0002-5040-4420
Uma AnandFaculty of Medicine, Imperial College London, Hammersmith Campus, Du Cane Road, London W12 0NN, U.K.
Elena V SviderskayaSchool of Health and Medical Sciences, City St George's, University of London, Cranmer Terrace, London SW17 0RE, U.K.
Joshua B EdelDepartment of Chemistry, Imperial College London, Molecular Sciences Research Hub, White City Campus, 82 Wood Lane, London W12 0BZ, U.K.ORCID https://orcid.org/0000-0001-5870-8659
Aleksandar P IvanovDepartment of Chemistry, Imperial College London, Molecular Sciences Research Hub, White City Campus, 82 Wood Lane, London W12 0BZ, U.K.ORCID https://orcid.org/0000-0003-1419-1381
Petr V GorelkinICAPPIC Limited, London NW10 6TD, U.K.
Yuri KorchevFaculty of Medicine, Imperial College London, Hammersmith Campus, Du Cane Road, London W12 0NN, U.K.
Andrew ShevchukFaculty of Medicine, Imperial College London, Hammersmith Campus, Du Cane Road, London W12 0NN, U.K.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Introducing exogenous biomolecules into individual cells with precise control over space, time, and dosage is crucial for both fundamental and applied biological research. Glass nanopipettes have long been employed to deliver biomolecules into individual cells; yet, their reliance on the electrical charge of the target molecule and the need for penetrating the cellular membrane pose significant limitations. We demonstrate that voltage pulses applied through a glass nanopipette in proximity to the cell membrane induce localized electroporation and generate directional flow, enabling controlled delivery of both charged and neutral biomolecules into subcellular compartments, e.g., the nucleus, without the need for penetrating the cellular membrane. This approach minimizes cell damage and preserves cell viability, even after multiple rounds of injection. Our findings will serve as a reference for the design of novel nanopipette methods, contributing to the newly established field of spatiotemporal analysis of live cells.

Indexed as

cell deliveryelectroporationInjectionnanoinjectionscanning-probe microscopySICMsingle-cell

Identifiers

PMID41112867
PMCPMC12531870

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.