Evidence map›Paper›PMID 41112792›Full record

ArticleBiomedical optics express2025

Two-dimensional nonlinear structured illumination microscopy with rsEGFP2.

Shaoheng Li, Ryo Tamura, Kota Banzai, Daichi Kamiyama, Peter Kner

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomedical optics express, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

5 authors.

Shaoheng LiSchool of Electrical and Computer Engineering, University of Georgia, Athens, GA, USA.
Ryo TamuraDepartment of Cellular Biology, University of Georgia, Athens, GA, USA.
Kota BanzaiDepartment of Cellular Biology, University of Georgia, Athens, GA, USA.
Daichi KamiyamaDepartment of Cellular Biology, University of Georgia, Athens, GA, USA.
Peter KnerSchool of Electrical and Computer Engineering, University of Georgia, Athens, GA, USA.ORCID https://orcid.org/0000-0001-7555-5180

Funding

Three-dimensional Nonlinear Structured Illumination for Live Imaging with 80 nm resolutionR01GM149978 · NIGMS · UNIVERSITY OF GEORGIA · PI Peter Alexander Kner · 2023 to 2026
$1.2M
Screening using split fluorescent protein tags for neurotransmitter receptors that define a synaptic balance in neuralcircuitsR21NS128750 · NINDS · UNIVERSITY OF GEORGIA · PI KAMIYAMA, DAICHI · 2023 to 2023
$404k
NIGMS NIH HHS R01 GM149978NINDS NIH HHS R21 NS128750
6 · The paper itself

Abstract

Nonlinear structured illumination microscopy has achieved resolutions down to 40 nm and imaging speeds up to 2.86 frames/s. However, relatively few realizations of NSIM have been published in the literature. Here, we demonstrate patterned depletion NSIM (PD-NSIM) with the fluorescent protein rsEGFP2. With its fast switching kinetics and large number of switching cycles, rsEGFP2 promises to be a useful fluorophore for NSIM, providing a combination of speed, resolution, and longevity that fills a gap in the imaging space. Here, we present the first demonstration of live 2D PD-NSIM using rsEGFP2. We demonstrate imaging of actin filaments in U2OS cells, achieving sub-80 nm resolution in live-cell imaging. We examine the images in both real-space and Fourier-space to support our claim of increased resolution.

Identifiers

PMID41112792
PMCPMC12532346

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.