ArticlePeerJ2025
rtpcr: a package for statistical analysis and graphical presentation of qPCR data in R.
Article in PeerJ, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
2 citing papers in PubMed.
- The CONSTANS-like gene family in wheat is involved in heading date and drought stress response.Scientific reports · 2025Article
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
1 author.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Background: Quantitative real-time polymerase chain reaction (qRT-PCR or qPCR) is widely used in molecular biology research. Various analysis methods are employed to interpret qPCR data and measure mRNA levels of target genes under different experimental conditions. Results: The rtpcr package was developed for amplification efficiency calculation, statistical analysis, and graphical presentation of qPCR data in R. It uses a general calculation methodology that accommodates up to two reference genes and amplification efficiency values, including the Pfaffl method. Depending on the experimental design, rtpcr functions apply a Conclusions: In summary, the rtpcr package is a useful and user-friendly tool for analyzing real-time PCR data from experiments involving up to three different factors. Built on a general methodology, it provides robust calculations and comprehensive graphical outputs, making it a valuable resource for researchers working with qPCR data.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.