ArticleFrontiers in cellular and infection microbiology2025
On-tissue derivatization for mass spectrometry imaging reveals the distribution of short chain fatty acids in murine digestive tract.
Article in Frontiers in cellular and infection microbiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers, 1 of them a synthesis that pooled it.
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Who cites it
3 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Mass spectrometry-based human spatial omics: fundamentals, innovations, and applications.Journal of biomedical science · 2026Pooled it
- Chemical Derivatization Strategies for Expanding Small Biomolecule Analysis by MALDI-MS.Metabolites · 2026Review
- Isolation and identification ofFrontiers in cellular and infection microbiology · 2026Article
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7 authors.
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Abstract
Short-chain fatty acids (SCFAs), which are produced by microorganisms in the digestive tract of animals, play an important role in maintaining homeostasis in the host, including immune function. Different types of SCFAs are produced by different intestinal bacterial communities. However, visualizing their spatial distribution within tissue sections has been difficult. This is primarily due to the volatility of SCFAs, which makes detection challenging, even with matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI) using an atmospheric pressure ion source. To address this issue, we minimized the volatility of SCFAs in fresh tissue sections. Then, we used N,N,N-trimethyl-2-(piperazin-1-yl)ethan-1-amine iodide (TMPA) and 1-((dimethylamino)(dimethylimino)methyl)-1H- [1,2,3]triazolo[4,5-b]pyridine-3-oxide hexafluorophosphate (HATU) to chemically derivatize the carboxylic acid into a quaternary amine. This
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