Evidence map›Paper›PMID 41112569›Full record

Observational studyFrontiers in cellular and infection microbiology2025

Multicenter evaluation of fast multiplex PCR for detection of pathogens in lower respiratory tract infections.

Ling Wang, Jingyao Cai, Lizhong Dai, Weimin Miao, Ziyang Li, Wei Cao, Sisong Huang, Mei Sun, Lehuan Xia, Xixin Jiang and 5 more

Abstract readMulticenter StudyObservational StudyComparative Study
PubMed Full text at PMC Publisher
In one paragraph

Observational study in Frontiers in cellular and infection microbiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Ling Wang *Department of Laboratory Medicine, The Second Xiangya Hospital of Central South University, Changsha, Hunan, China.
Jingyao Cai *Department of Laboratory Medicine, The Second Xiangya Hospital of Central South University, Changsha, Hunan, China.
Lizhong DaiSansure Biotech Inc., Changsha, Hunan, China.
Weimin MiaoSansure Biotech Inc., Changsha, Hunan, China.
Ziyang LiDepartment of Laboratory Medicine, The Second Xiangya Hospital of Central South University, Changsha, Hunan, China.
Wei CaoDepartment of Laboratory Medicine, The Second Xiangya Hospital of Central South University, Changsha, Hunan, China.
Sisong HuangSansure Biotech Inc., Changsha, Hunan, China.
Mei SunDepartment of Laboratory Medicine, The Second Xiangya Hospital of Central South University, Changsha, Hunan, China.
Lehuan XiaDepartment of Laboratory Medicine, Chenzhou Third People's Hospital, Chenzhou, Hunan, China.
Xixin JiangDepartment of Laboratory Medicine, Yueyang People's Hospital, Yueyang, Hunan, China.
Pingbang WangDepartment of Laboratory Medicine, The People's Hospital of Liuyang, Liuyang, Hunan, China.
Jianhua PanDepartment of Laboratory Medicine, Changsha Central Hospital, Changsha, Hunan, China.
Qinghe YangDepartment of Laboratory Medicine, The Second People's Hospital of Hunan Province Huaihua City, Huaihua, Hunan, China.
Anqun YangDepartment of Laboratory Medicine, The Second People's Hospital of Hunan Province Huaihua City, Huaihua, Hunan, China.
Min HuDepartment of Laboratory Medicine, The Second Xiangya Hospital of Central South University, Changsha, Hunan, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Background: This study aimed to compare the diagnostic performance between bacterial culture and a rapid multiplex PCR assay (Respiratory Pathogens Multiplex Nucleic Acid Diagnostic Kit) for detecting six bacterial (Pseudomonas aeruginosa, Klebsiella pneumoniae, Staphylococcus aureus, Streptococcus pneumoniae, Haemophilus influenzae, Legionella pneumophila) and six viral targets (Influenza A/B, Respiratory syncytial virus, Adenoviruses, Human rhinovirus, Mycoplasma pneumoniae) in 728 bronchoalveolar lavage (BAL) specimens. Methods: This multicentric observational study was conducted at six comprehensive large hospitals in Hunan Province, China (May to October 2023) and assessed performance of mPCR kit by comparison with conventional culture method. Results: The mPCR kit detected ≥1 pathogen in 628 specimens (positivity rate: 86.3%), demonstrating positive percentage agreement (PPA) of 84.6% (95% CI: 76.6-92.6%) and negative percentage agreement (NPA) of 96.5% (95% CI: 96.0-97.1%) versus culture. Notably, semi-quantitative concordance was 79.3% (283/357) for culture-positive specimens. Multiple pathogens were detected by mPCR in 144 samples (19.8%). Lower Ct values (≤30) correlated strongly with culture positivity. Conclusions: The mPCR kit is a simple and rapid molecular test that could complement conventional culture method for improvement of diagnosis accuracy of Lower respiratory tract infections.

Indexed as

BacteriaMolecular Diagnostic TechniquesMultiplex Polymerase Chain ReactionRespiratory Tract InfectionsVirus DiseasesVirusesAdolescentAdultAgedAged, 80 and overBronchoalveolar Lavage FluidChildChild, PreschoolChinaFemaleHumanslower respiratory tract infectionsmulticenter evaluationmultiplex PCRpathogen detectionsemi-quantitative result

Identifiers

PMID41112569
PMCPMC12528164

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.