ArticleJournal of biomedical optics2025
Multimodal microscope for optical coherence microscopy, tomography, vibrometry, and two-photon microscopy in the living mouse cochlea.
Article in Journal of biomedical optics, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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6 authors.
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Abstract
Significance: Our understanding of mechanotransduction in mammalian inner ears remains incomplete, in part due to imaging limitations: current systems cannot simultaneously provide high-resolution images needed for subcellular analysis and the deep focus required for structural mechanics. Optical coherence tomography (OCT) enables structural and vibrational imaging through the bone of the intact cochlea in models such as mice, supporting studies of cochlear mechanics in animals with functional hearing. However, capturing both cellular ( Aim: We aim to develop an integrated OCT and two-photon microscope optimized for imaging the morphology and function of the cochlea. Approach: We integrated a custom SD-OCT/TPM system into an upright microscope with a high-precision stage for animal positioning. The system uses two tunable liquid lenses to form a beam expander, enabling dynamic adjustment of the beam diameter at the back aperture of each objective. This optimized light throughput and maintained a high signal-to-noise ratio (SNR) across all objectives. In addition, we automated optical adjustments to facilitate seamless imaging with a wide range of objectives. Results: For each objective, we measured the SNR difference between a beam expanded to match the largest back aperture and a beam adjusted to match the back aperture of the objective. Except for the Conclusions: Automation and optimization of the optical system enabled seamless multiscale imaging of the murine cochlea, providing high-quality morphological, functional, and two-photon fluorescence images. The dynamic adjustment of the beam diameter within the microscope was essential for maintaining high SNR across a wide range of numerical apertures.
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