Evidence map›Paper›PMID 41110981›Full record

ArticleThe Journal of physiology2025

VasoTracker 2: Open-source software and hardware for tracking blood vessel diameter and assessing vascular function.

Matthew D Lee, Christopher Osborne, Ross Stevenson, Amy MacDonald, Grace Ebner, Danielle A Jeffrey, Margaret A MacDonald, Xun Zhang, Charlotte Buckley, Fabrice Dabertrand and 6 more

Abstract read
In one paragraph

Article in The Journal of physiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Guidelines for evaluating endothelial function in vascular tissue.American journal of physiology. Heart and circulatory physiology · 2026
    Review
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors.

Matthew D LeeStrathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, Glasgow, UK.ORCID 0000-0001-8265-382X
Christopher OsborneFaculty of Science, University of Strathclyde, Strathclyde, UK.ORCID 0000-0002-2299-2800
Ross StevensonStrathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, Glasgow, UK.
Amy MacDonaldStrathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, Glasgow, UK.
Grace EbnerDepartment of Pharmacology, Larner College of Medicine, Vermont Center for Cardiovascular and Brain Health, University of Vermont, Burlington, VT, USA.ORCID 0009-0005-5880-8154
Danielle A JeffreyDepartment of Anesthesiology, University of Colorado Anschutz Medical Campus, Aurora, CO, USA.ORCID 0000-0002-9576-9458
Margaret A MacDonaldStrathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, Glasgow, UK.
Xun ZhangStrathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, Glasgow, UK.ORCID 0000-0003-0790-4291
Charlotte BuckleyStrathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, Glasgow, UK.ORCID 0000-0002-7961-4544
Fabrice DabertrandDepartment of Anesthesiology, University of Colorado Anschutz Medical Campus, Aurora, CO, USA.ORCID 0000-0003-2541-9185
Daniel R MachinDepartment of Cell Biology and Physiology, University of New Mexico School of Medicine, Albuquerque, NM, USA.
Jason AuDepartment of Kinesiology and Health Sciences, University of Waterloo, Waterloo, ON, Canada.ORCID 0000-0001-8601-323X
Osama F HarrazDepartment of Pharmacology, Larner College of Medicine, Vermont Center for Cardiovascular and Brain Health, University of Vermont, Burlington, VT, USA.ORCID 0000-0003-2061-1100
Nathan TykockiDepartment of Pharmacology and Toxicology, Michigan State University, East Lansing, MI, USA.ORCID 0000-0001-5432-7656
John G McCarronStrathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, Glasgow, UK.ORCID 0000-0002-3302-3984
Calum WilsonStrathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, Glasgow, UK.ORCID 0000-0003-2500-0632

Funding

SIGNALING MECHANISMS IN ARTERIES AND VEINS IN HYPERTENSIONP01HL070687 · NHLBI · MICHIGAN STATE UNIVERSITY · PI FINK, GREGORY D · 2003 to 2019
$22.8M
Vermont Center for Cardiovascular and Brain HealthP20GM135007 · NIGMS · UNIVERSITY OF VERMONT & ST AGRIC COLLEGE · PI Diego Adrianzen Herrera · 2020 to 2026
$17.4M
The perivascular adipocyte: integrating mechanical and structural cues into vasoactive functionP01HL152951 · NHLBI · MICHIGAN STATE UNIVERSITY · PI Stephanie W Watts · 2022 to 2026
$13.3M
PREDOCTORAL TRAINING PROGRAM IN PHARMACOLOGYT32GM007635 · NIGMS · UNIVERSITY OF COLORADO DENVER · PI DELL'ACQUA, MARK L · 1985 to 2023
$6.1M
Capillary control of cerebral blood flow, and its disruption in small vessel diseaseR01HL136636 · NHLBI · UNIVERSITY OF VERMONT & ST AGRIC COLLEGE · PI Fabrice Dabertrand · 2017 to 2026
$3.8M
Brain capillary endothelial cell energetics and neurovascular uncoupling in dementiaRF1NS140137 · NINDS · UNIVERSITY OF COLORADO DENVER · PI DABERTRAND, FABRICE, GARCIA, ANASTACIA M · 2024 to 2024
$2.4M
Endothelial Piezo1 channel and cerebral blood flow controlR01HL169681 · NHLBI · UNIVERSITY OF VERMONT & ST AGRIC COLLEGE · PI Osama F Harraz · 2023 to 2026
$2.3M
TRPV1 Mediates Progressive Stress-Induced Bladder DysfunctionR01DK119615 · NIDDK · MICHIGAN STATE UNIVERSITY · PI MINGIN, GERALD, TYKOCKI, NATHAN ROGER · 2019 to 2023
$1.9M
Rescuing neurovascular coupling to protect neuronal plasticity and cognitionRF1NS129022 · NINDS · UNIVERSITY OF COLORADO DENVER · PI DABERTRAND, FABRICE, DELL'ACQUA, MARK L · 2022 to 2022
$1.8M
Bladder Wall Stiffness Drives Sensation of FullnessR01DK135696 · NIDDK · HENRY FORD HEALTH + MICHIGAN STATE UNIVERSITY HEALTH SCIENCES · PI Sara Roccabianca, Nathan Roger Tykocki · 2024 to 2026
$1.6M
The role of hyaluronan in age-related vascular and skeletal muscle dysfunctionR00AT010017 · NCCIH · FLORIDA STATE UNIVERSITY · PI MACHIN, DANIEL ROBERT · 2020 to 2022
$747k
Brain capillary Piezo1 ion channels and blood flow regulation in Alzheimer’s DiseaseR21AG082193 · NIA · UNIVERSITY OF MIAMI CORAL GABLES · PI BRACKO, OLIVER, HARRAZ, OSAMA F · 2023 to 2024
$434k
American Heart Association-American Stroke Association 20CDA35310097 - OSAMA HARRAZNCCIH NIH HHS R00 AT010017NHLBI NIH HHS F31 HL170645NHLBI NIH HHS P01 HL070687NHLBI NIH HHS P01 HL152951NHLBI NIH HHS R01 HL136636NHLBI NIH HHS R01 HL169681NIA NIH HHS R21 AG082193NIDDK NIH HHS R01 DK119615NIDDK NIH HHS R01 DK135696NIGMS NIH HHS P20 GM135007NIGMS NIH HHS T32 GM007635NINDS NIH HHS RF1 NS129022NINDS NIH HHS RF1 NS140137Wellcome Trust
6 · The paper itself

Abstract

VasoTracker 2 is a collection of open-source tools for blood vessel diameter measurement and vascular physiology research, featuring automated diameter tracking software and low-cost myography hardware components. This blood vessel analysis platform addresses the need for accessible alternatives to expensive commercial vascular imaging systems by providing comprehensive tools for studying vessel reactivity and endothelial function. The software enables multipoint diameter tracking in branched vessels using advanced edge detection algorithms, supporting brightfield microscopy, fluorescence imaging and ultrasound recordings. Automated pressure protocols enable standardized myogenic tone experiments, and both real-time and offline analysis of pre-recorded data are supported. The platform's versatility allows researchers to study vascular dynamics across diverse experimental conditions, from isolated vessel preparations to in vivo imaging applications. For ex vivo applications, VasoTracker 2 includes modular, low-cost open-source pressure myograph hardware: a confocal-compatible vessel chamber and a programmable pressure controller, VasoMoto. These components enable construction of a complete pressure myograph system at a significantly reduced cost compared to commercial alternatives. The hardware integrates seamlessly with the diameter measurement software.VasoTracker 2 provides free software and low-cost hardware for vessel diameter analysis, broadening access to advanced vascular research tools. The platform's open-source nature allows researchers to modify and extend the system for specific applications. This flexibility, combined with significant cost savings, benefits vascular researchers worldwide. KEY POINTS: VasoTracker 2 is an open-source platform that combines versatile vessel diameter tracking software with modular low-cost hardware components for vascular physiology research. The software enables multipoint diameter analysis across brightfield, fluorescence and ultrasound imaging modalities. The hardware includes a confocal-compatible vessel chamber and programmable pressure controller (VasoMoto) that can be assembled into a complete pressure myograph system. The open-source approach provides researchers with accessible tools for advanced vascular physiology experiments.

Indexed as

Blood VesselsMyographySoftwareAnimalsHumansblood vessel diameterendotheliumopen sourcesmooth musclevasoconstrictionvasodilatation

Identifiers

PMID41110981
PMCPMC12645537

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.