Evidence map›Paper›PMID 41109683›Full record

ArticleThe Plant cell2025

Rational design of induced regeneration via somatic embryogenesis in the absence of exogenous phytohormones.

Jana Wittmer, Menno Pijnenburg, Tristan Wijsman, Sieme Pelzer, Kelvin Adema, Merijn Kerstens, An-Nikol Kutevska, Joke Fierens, Hugo Hofhuis, Robert Sevenier and 6 more

Abstract read
In one paragraph

Article in The Plant cell, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed.

  1. Article
  2. Review
  3. Review
  4. Review
  5. Article
  6. Review
  7. Article
  8. Article
  9. From Lab to Field: CRISPRing Major CultivatedInternational journal of molecular sciences · 2026
    Review
  10. Genome-Wide Identification and Expression Analysis of theInternational journal of molecular sciences · 2026
    Article
  11. Review
  12. Review
  13. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors.

Jana WittmerCell and Developmental Biology, Cluster Plant Developmental Biology, Wageningen University & Research, Droevendaalsesteeg 1, Wageningen 6708PB, The Netherlands.ORCID 0009-0004-6575-4724
Menno PijnenburgCell and Developmental Biology, Cluster Plant Developmental Biology, Wageningen University & Research, Droevendaalsesteeg 1, Wageningen 6708PB, The Netherlands.ORCID 0009-0000-7444-0584
Tristan WijsmanCell and Developmental Biology, Cluster Plant Developmental Biology, Wageningen University & Research, Droevendaalsesteeg 1, Wageningen 6708PB, The Netherlands.ORCID 0009-0007-9039-9260
Sieme PelzerCell and Developmental Biology, Cluster Plant Developmental Biology, Wageningen University & Research, Droevendaalsesteeg 1, Wageningen 6708PB, The Netherlands.ORCID 0009-0007-3763-8277
Kelvin AdemaCell and Developmental Biology, Cluster Plant Developmental Biology, Wageningen University & Research, Droevendaalsesteeg 1, Wageningen 6708PB, The Netherlands.ORCID 0009-0005-6892-1345
Merijn KerstensCell and Developmental Biology, Cluster Plant Developmental Biology, Wageningen University & Research, Droevendaalsesteeg 1, Wageningen 6708PB, The Netherlands.ORCID 0000-0003-4131-1721
An-Nikol KutevskaCell and Developmental Biology, Cluster Plant Developmental Biology, Wageningen University & Research, Droevendaalsesteeg 1, Wageningen 6708PB, The Netherlands.ORCID 0009-0003-7784-894X
Joke FierensKeygene N.V., Agro Business Park 90, Wageningen 6708 PW, The Netherlands.ORCID 0009-0006-2883-1959
Hugo HofhuisKeygene N.V., Agro Business Park 90, Wageningen 6708 PW, The Netherlands.
Robert SevenierKeygene N.V., Agro Business Park 90, Wageningen 6708 PW, The Netherlands.ORCID 0009-0000-0222-0603
Bjorn KloostermanKeygene N.V., Agro Business Park 90, Wageningen 6708 PW, The Netherlands.ORCID 0009-0000-4250-9874
Michiel de BothKeygene N.V., Agro Business Park 90, Wageningen 6708 PW, The Netherlands.ORCID 0009-0003-1818-5162
Wouter KohlenCell and Developmental Biology, Cluster Plant Developmental Biology, Wageningen University & Research, Droevendaalsesteeg 1, Wageningen 6708PB, The Netherlands.ORCID 0000-0001-9057-2392
Harm NijveenBioinformatics Group, Wageningen University & Research, Droevendaalsesteeg 1, Wageningen 6708PB, The Netherlands.ORCID 0000-0002-9167-4945
Ben ScheresMolecular Biology, Cluster Plant Developmental Biology, Wageningen University & Research, Droevendaalsesteeg 1, Wageningen 6708PB, The Netherlands.ORCID 0000-0001-5400-9578
Renze HeidstraCell and Developmental Biology, Cluster Plant Developmental Biology, Wageningen University & Research, Droevendaalsesteeg 1, Wageningen 6708PB, The Netherlands.ORCID 0000-0001-9032-5770

Funding

BASFBejo Zaden B.V.Dutch Research CouncilEnza Zaden Research & Development B.V.Keygene NVLimagrainNWO-TKI Topsector TU202208NWO-TTW LettuceKnowRijk Zwaan Breeding B.V.Syngenta Seeds B.V.Takii and Company Ltd. GSGT.2019.019VIDI VI.Vidi.193.119
6 · The paper itself

Abstract

Plants have a remarkable regenerative capacity, but this varies widely among species and tissue types. Important crop cultivars show regenerative recalcitrance, which is a major obstacle for the application of modern plant propagation and breeding techniques. Regeneration generally involves empirically determined tissue culture methods that are based on the principle of inducing totipotency. Cells are first persuaded to change fate toward root stem cell-like identity and then are reprogrammed to acquire shoot fate. Alternatively, pluri- or totipotent cells can lead to the formation of a complete plantlet through somatic embryogenesis. We applied our knowledge of root stem cell niche biology to directly use the implicated stem cell factors, including RETINOBLASTOMA (RBR), SCARECROW (SCR), SHORT ROOT (SHR), and members of the AINTEGUMENTA-LIKE/PLETHORA (AIL/PLT) and WUSCHEL-related homeobox (WOX) gene families, as a tool to induce regeneration in a way similar to the principle of induced pluripotent stem cells in the animal field. We show that stem cell factors synergistically induce regeneration involving the somatic embryogenesis pathway and can break recalcitrance in Arabidopsis (Arabidopsis thaliana) and pepper (Capsicum annuum).

Indexed as

ArabidopsisPlant Growth RegulatorsPlant Somatic Embryogenesis TechniquesRegenerationArabidopsis ProteinsGene Expression Regulation, PlantPlant RootsArabidopsis ProteinsPlant Growth Regulators

Identifiers

PMID41109683
PMCPMC12586336

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.