Evidence map›Paper›PMID 41108608›Full record

ArticleJournal of clinical laboratory analysis2025

Quantitative Analysis of DNA Double-Strand Breaks in Genomic DNA Using Standard Curve Method.

Lihuang Guo, Hanying Dai, Jiancheng Li, Chenwei Li, Yue Huang, Keqian Xu

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of clinical laboratory analysis, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Lihuang GuoDepartment of Laboratory Medicine, the Third Xiangya Hospital, Central South University, Changsha, China.ORCID https://orcid.org/0000-0002-0946-5643
Hanying DaiDepartment of Laboratory Medicine, the Third Xiangya Hospital, Central South University, Changsha, China.
Jiancheng LiDepartment of Laboratory Medicine, the Third Xiangya Hospital, Central South University, Changsha, China.
Chenwei LiDepartment of Laboratory Medicine, the Third Xiangya Hospital, Central South University, Changsha, China.
Yue HuangDepartment of Laboratory Medicine, the Third Xiangya Hospital, Central South University, Changsha, China.
Keqian XuDepartment of Laboratory Medicine, the Third Xiangya Hospital, Central South University, Changsha, China.

Funding

Wu Jieping Medical Foundation 320.6750.2024-6-133
6 · The paper itself

Abstract

backgroundDNA double-strand breaks (DSBs) are the most lethal and dangerous type of lesions with significant implications for both cellular function and organismal health. The number of DSBs (N

methodGenomic DNA from human, mouse, Arabidopsis, Saccharomyces cerevisiae, and Escherichia coli was digested by seven blunt-end restriction enzymes to generate DSB standards. Theoretical N

resultsAll genomes were successfully digested by seven blunt-end restriction enzymes to produce standard DSB fragments. Standard curves demonstrated high linearity (R

conclusionThis standard curve-based method enables accurate, reproducible quantification of genome-wide DSBs in various organisms. It is simple, low-cost, and easily standardized, offering a promising tool for applications in genotoxicity testing, environmental exposure monitoring, and DNA damage research.

Indexed as

DNADNA Breaks, Double-StrandedReal-Time Polymerase Chain ReactionAnimalsArabidopsisEscherichia coliHumansMiceReproducibility of ResultsSaccharomyces cerevisiaeDNADNA damageDNA double‐strand breaksligation‐mediated real‐time quantitative PCRstandard curve method

Identifiers

PMID41108608
PMCPMC12699195

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.