ArticlePhysiological reports2025
The pH-dependent photophysical and spectral properties of pH-sensing green fluorescent proteins.
Article in Physiological reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 1 paper.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
1 citing paper in PubMed.
- The pH-dependent photophysical and spectral properties of pH-sensing green fluorescent proteins.Physiological reports · 2025Article
Corrections and comments
- Erratum issued
Authors and funding
1 author.
Funding
Abstract
Protonation and deprotonation reactions significantly influence biological function, with buffers and transporters maintaining stable pH levels in the cytosol and various organs. However, pH can vary considerably in organ lumens, such as the airway surface liquid (ASL), which serves as a defense barrier for airways. Traditional microelectrodes, while precise, face limitations prompting the use of fluorescent pH sensors. Although many green fluorescent protein (GFP)-based pH sensors have been reported, data are usually limited to their spectral properties. Photophysical properties are often reported for a single pH value. In this study, the photophysical properties of pHluorins and superfolder GFP-including a novel pH sensor pHluorin4-were compared. Purified pHluorin4 effectively reported ASL pH differences in cystic fibrosis (CF) and non-CF epithelia. These findings provide a comprehensive summary of the photophysical and spectral properties of popular pHluorins, including the new member pHluorin4, which can be used to optimize their application in different systems.
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Registered trials
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