Evidence map›Paper›PMID 41108374›Full record

ArticleApplied microbiology and biotechnology2025

A chemiluminescence immunoassay for detecting neutralizing antibodies of foot-and-mouth disease virus serotype A.

Mengyang Sun, Yanfang Bao, Kun Li, Yili Zuo, Huiyan Zhang, Yuanfang Fu, Pinghua Li, Pu Sun, Zhixun Zhao, Tao Jiang and 4 more

Abstract readEvaluation Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Applied microbiology and biotechnology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Mengyang SunState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, National Foot-and-Mouth Diseases Reference Laboratory, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Xujiaping No. 1, Yanchangpu, Lanzhou, Gansu, 730046, China.
Yanfang BaoLanzhou Shouyan Biotechnology Co., Ltd, Lanzhou, 730046, China.
Kun LiState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, National Foot-and-Mouth Diseases Reference Laboratory, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Xujiaping No. 1, Yanchangpu, Lanzhou, Gansu, 730046, China.
Yili ZuoLanzhou Shouyan Biotechnology Co., Ltd, Lanzhou, 730046, China.
Huiyan ZhangState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, National Foot-and-Mouth Diseases Reference Laboratory, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Xujiaping No. 1, Yanchangpu, Lanzhou, Gansu, 730046, China.
Yuanfang FuState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, National Foot-and-Mouth Diseases Reference Laboratory, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Xujiaping No. 1, Yanchangpu, Lanzhou, Gansu, 730046, China.
Pinghua LiState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, National Foot-and-Mouth Diseases Reference Laboratory, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Xujiaping No. 1, Yanchangpu, Lanzhou, Gansu, 730046, China.
Pu SunState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, National Foot-and-Mouth Diseases Reference Laboratory, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Xujiaping No. 1, Yanchangpu, Lanzhou, Gansu, 730046, China.
Zhixun ZhaoState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, National Foot-and-Mouth Diseases Reference Laboratory, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Xujiaping No. 1, Yanchangpu, Lanzhou, Gansu, 730046, China.
Tao JiangLanzhou Shouyan Biotechnology Co., Ltd, Lanzhou, 730046, China.
Xingwen BaiState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, National Foot-and-Mouth Diseases Reference Laboratory, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Xujiaping No. 1, Yanchangpu, Lanzhou, Gansu, 730046, China.
Mi LinLanzhou Shouyan Biotechnology Co., Ltd, Lanzhou, 730046, China. linmi@caas.cn.
Zengjun LuState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, National Foot-and-Mouth Diseases Reference Laboratory, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Xujiaping No. 1, Yanchangpu, Lanzhou, Gansu, 730046, China. luzengjun@caas.cn.
Yimei CaoState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, National Foot-and-Mouth Diseases Reference Laboratory, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Xujiaping No. 1, Yanchangpu, Lanzhou, Gansu, 730046, China. caoyimei@caas.cn.

Funding

National Key Research and Development Program of China 2021YFD1800300Talent Innovation and Entrepreneurship Project of lanzhou 2024-HL-7The Major Science and Technology Project of Gansu Province 23ZDNA007
6 · The paper itself

Abstract

Foot-and-mouth disease (FMD) is a highly contagious viral disease affecting cloven-hoofed animals, posing significant threats to global livestock industries. Post-vaccination neutralizing antibody levels reflect vaccine efficacy, but traditional virus neutralization tests (VNT) for detecting neutralizing antibodies requires restrictive biocontainment facilities to handle live virus, is time-consuming and laborious. In this study, we successfully established a high-efficiency magnetic particle-based chemiluminescence immunoassay (MP-CLIA) using two previously characterized monoclonal antibodies (W125 and W145) generated through single B cell antibody technology. The MP-CLIA method exhibited a sensitivity of 95.93% and specificity of 100%, with a cut-off value of 41.395 activity units (U) by detecting the known 221 positive and 122 negative sera. The positive/negative coincidence rate between the MP-CLIA and VNT was 92.2% and the kappa coefficient was 78.19%, indicating a relatively high level of consistency. When integrated with a fully automated chemiluminescence immunoassay analyzer, this method only takes 20 min for a single test. These results show that MP-CLIA is a promising tool for the safe, rapid, and fully automatic detection of neutralizing antibodies against FMD virus (FMDV) serotype A. KEY POINTS: A MP-CLIA using two bovine single B cell antibodies was established. The MP-CLIA method exhibited a sensitivity of 95.93% and specificity of 100%. The MP-CLIA specifically detects neutralizing antibodies against FMDV serotype A.

Indexed as

Antibodies, NeutralizingAntibodies, ViralFoot-and-Mouth DiseaseFoot-and-Mouth Disease VirusLuminescent MeasurementsAnimalsAntibodies, MonoclonalCattleImmunoassayNeutralization TestsSensitivity and SpecificitySerogroupAntibodies, MonoclonalAntibodies, NeutralizingAntibodies, ViralChemiluminescence immunoassayFoot-and-mouth disease virusMagnetic particleNeutralizing antibodiesSerotype A

Identifiers

PMID41108374
PMCPMC12535544

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LicenceCC BY-NC-ND
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.