Evidence map›Paper›PMID 41108014›Full record

ArticleEuropean journal of medical research2025

Adipocytes promoted anoikis resistance of head and neck cancer cells via overexpressed ITGA5.

Yurong He, Yueyue Shi, Xinyu Li, Ru Wang, Lingwa Wang, Haiyang Li, Siyu Zhu, Shizhi He, Jugao Fang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in European journal of medical research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Yurong HeDepartment of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, No.1, Dongjiaominxiang Street, Dongcheng District, Beijing, 100730, People's Republic of China.
Yueyue ShiDepartment of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, No.1, Dongjiaominxiang Street, Dongcheng District, Beijing, 100730, People's Republic of China.
Xinyu LiDepartment of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, No.1, Dongjiaominxiang Street, Dongcheng District, Beijing, 100730, People's Republic of China.
Ru WangDepartment of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, No.1, Dongjiaominxiang Street, Dongcheng District, Beijing, 100730, People's Republic of China.
Lingwa WangDepartment of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, No.1, Dongjiaominxiang Street, Dongcheng District, Beijing, 100730, People's Republic of China.
Haiyang LiDepartment of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, No.1, Dongjiaominxiang Street, Dongcheng District, Beijing, 100730, People's Republic of China.
Siyu ZhuDepartment of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, No.1, Dongjiaominxiang Street, Dongcheng District, Beijing, 100730, People's Republic of China.
Shizhi HeDepartment of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, No.1, Dongjiaominxiang Street, Dongcheng District, Beijing, 100730, People's Republic of China. hsz_bjtr@mail.ccmu.edu.cn.
Jugao FangDepartment of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, No.1, Dongjiaominxiang Street, Dongcheng District, Beijing, 100730, People's Republic of China. fangjugao2@ccmu.edu.cn.

Funding

Industry-University-Research Innovation Fund for Chinese Universities of Ministry of Education of China No. 2024GR059
6 · The paper itself

Abstract

backgroundHead and neck squamous cell carcinoma (HNSCC) was the sixth most common cancer worldwide. Given the evidence that adipocytes are related to the progression of various cancers, this study explored the internal connection and mechanism between HNSCC and adipocytes.

methodsQuantitative real-time PCR (qRT-PCR) was used to examine the transfection efficiency of integrin subunit α5 (ITGA5) overexpression plasmid and small interfering RNA of ITGA5 (si-ITGA5). Colony formation assay was employed to detect the colony formation rate of FaDu and SAS cells, while flow cytometry was used to measure cell apoptosis rate. The protein levels of cleaved-caspase-3 (apoptosis marker) and cell adhesion markers (ITGA5, integrin subunit β1 (ITGB1) and cluster of differentiation 44 (CD44)) were analyzed by western blotting. After HNSCC cells and adipocytes were co-cultured, the last three experiments above-mentioned were repeated and Calcein AM/EthD-1 double stain assay was performed to examine anoikis.

resultsOverexpressed ITGA5 enhanced colony formation and inhibited anoikis in HNSCC cells (p < 0 p < .001), while reducing the expression of the apoptosis-related protein and increasing those of adherence-related proteins (p < 0.05). However, si-ITGA5 had the opposite effect. After co-culture of HNSCC cells with adipocytes, cell colony formation was increased, anoikis was inhibited and the expressions of ITGA5 and cell adhesion markers were upregulated (p < 0.05). The effects of HNSCC cells co-cultured with adipocytes on colony formation, anoikis and cell adhesion markers were reversed by ITGA5 silencing (p < 0.01).

conclusionAdipocytes promote anoikis resistance of head and neck cancer cells via overexpressed ITGA5.

Indexed as

AdipocytesAnoikisHead and Neck NeoplasmsIntegrin alpha5Squamous Cell Carcinoma of Head and NeckApoptosisCell AdhesionCell Line, TumorGene Expression Regulation, NeoplasticHumansIntegrinsIntegrin alpha5IntegrinsITGA5 protein, humanAdipocytesAnoikisHead and neck squamous cell carcinomaIntegrin subunit α5

Identifiers

PMID41108014
PMCPMC12535057

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.