Evidence map›Paper›PMID 41107732›Full record

ArticleBMC plant biology2025

Revealing the genetic diversity and population structure in lentil (Lens culinaris) germplasm using inter-primer binding site (iPBS)-retrotransposon markers.

Sarmad Ali Qureshi, Meliha Feryal Sarıkaya, Muhammad Azhar Nadeem, Amjad Ali, Parnaz Mortazavi, Mehmet Bedir, Muhammed Tatar, Aigul Madenova, Balnur Kabylbekova, Fawad Ali and 6 more

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Article in BMC plant biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

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2citing papers in PubMed
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1 · What the graph read from it

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3 · Its place in the literature

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2 citing papers in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

16 authors.

Sarmad Ali QureshiFaculty of Agricultural Sciences and Technologies, Sivas University of Science and Technology, Sivas, 58140, Türkiye.
Meliha Feryal SarıkayaFaculty of Agricultural Sciences and Technologies, Sivas University of Science and Technology, Sivas, 58140, Türkiye.
Muhammad Azhar NadeemDepartment of Biotechnology, Faculty of Science, Mersin University, Mersin, 33343, Türkiye. azharjoiya22@gmail.com.
Amjad AliFaculty of Agricultural Sciences and Technologies, Sivas University of Science and Technology, Sivas, 58140, Türkiye.
Parnaz MortazaviFaculty of Agricultural Sciences and Technologies, Sivas University of Science and Technology, Sivas, 58140, Türkiye.
Mehmet BedirFaculty of Agricultural Sciences and Technologies, Sivas University of Science and Technology, Sivas, 58140, Türkiye.
Muhammed TatarFaculty of Agricultural Sciences and Technologies, Sivas University of Science and Technology, Sivas, 58140, Türkiye.
Aigul MadenovaKazakh Fruit and Vegetable Research Institute, Almaty, 050060, Kazakhstan. madenova.a@mail.ru.
Balnur KabylbekovaKazakh Fruit and Vegetable Research Institute, Almaty, 050060, Kazakhstan.
Fawad AliSchool of Breeding and Multiplication (Sanya Institute of Breeding and Multiplication), School of Tropical Agriculture and Forestry, Hainan University, Sanya, Hainan, 572025, China.
Muhammad Tanveer AltafDepartment of Field Crops, Faculty of Agriculture, Recep Tayyip Erdoğan University, Rize/Pazar, Türkiye.
Sagi SoltanbekovKazakh Fruit and Vegetable Research Institute, Almaty, 050060, Kazakhstan.
Sati TokibayevLLP Research Center for Bio and IT Technologies, Almaty, Kazakhstan.
Muhammad Kashif IlyasNational Agricultural Research Centre, Park Road, Islamabad, 45500, Pakistan.
Kağan KöktenFaculty of Agricultural Sciences and Technologies, Sivas University of Science and Technology, Sivas, 58140, Türkiye.
Faheem Shahzad BalochDepartment of Biotechnology, Faculty of Science, Mersin University, Mersin, 33343, Türkiye.

Funding

Ministry of Agriculture of the Republic of Kazakhstan BR22885401
6 · The paper itself

Abstract

backgroundLentil (L. culinaris ssp. culinaris) is a climate-resilient legume crop that plays a key role in global food security and sustainable agriculture. Understanding genetic diversity and population structure is essential for conserving genetic resources, enhancing breeding strategies, and developing improved, high-performing cultivars.

resultsThe genetic diversity and population structure of 96 lentil genotypes were evaluated using 15 iPBS-retrotransposon markers, which produce a total of 346 scorable bands. Among these primers, iPBS-2376 yielded the highest number of bands (33). The polymorphism information content (PIC) values ranged from 0.17 to 0.38, indicating a broad range of marker informativeness. Genetic diversity analysis revealed a mean gene diversity (h) of 0.29, a Shannon information index (I) of 0.44, and an effective number of alleles (Ne) of 1.48. The maximum genetic distance (0.88) was observed between genotypes G25 (Pakistan25) and G94 (Cagil), reflecting this genetic divergence. Analysis of molecular variance (AMOVA) revealed that 82% of the total variation occurred within populations. The STRUCTURE analysis and neighbor-joining (NJ) clustering consistently grouped the genotypes into two distinct clusters, largely corresponding to their geographic origins. Similarly, Principal Coordinate Analysis (PCoA) further confirmed this separation based on geographic distribution.

conclusionsThese results demonstrate considerable genetic diversity within the lentil germplasm. This suggests that these accessions can be employed in breeding programs to design improved lentil cultivars with enhanced resilience, higher crop output, and broader adaptability.

Indexed as

Genetic VariationLens PlantRetroelementsDNA PrimersGenetic MarkersGenotypePhylogenyDNA PrimersGenetic MarkersRetroelementsGenetic diversity assessmentGermplasm characterizationIPBS-retrotransposon markersLentilMolecular breeding resourcesPopulation structure analysis

Identifiers

PMID41107732
PMCPMC12534935

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.