Evidence map›Paper›PMID 41106724›Full record

ArticleJournal of advanced research2026

Exosomes derived from M1-macrophage promote enteric neuronal injury via MMP8-TGF-β pathway.

Yuqiong Chen, Kai Song, Yi Zheng, Chaoting Lan, Yide Mu, Zuyi Ma, Gen Chen, Yun Zhu, Yongwei Huang, Jiazhang Chen and 8 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of advanced research, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Review
  2. Review
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

18 authors.

Yuqiong ChenProvincial Key Laboratory of Research in Structure Birth Defect Disease and Department of Pediatric Surgery, Guangzhou Women and Children's Medical Center, Guangzhou Medical University, Guangzhou 510623 Guangdong, China; Department of Pediatrics, Guangzhou Eighth People's Hospital, Guangzhou Medical University, Guangzhou 510623 Guangdong, China.
Kai SongProvincial Key Laboratory of Research in Structure Birth Defect Disease and Department of Pediatric Surgery, Guangzhou Women and Children's Medical Center, Guangzhou Medical University, Guangzhou 510623 Guangdong, China.
Yi ZhengProvincial Key Laboratory of Research in Structure Birth Defect Disease and Department of Pediatric Surgery, Guangzhou Women and Children's Medical Center, Guangzhou Medical University, Guangzhou 510623 Guangdong, China.
Chaoting LanProvincial Key Laboratory of Research in Structure Birth Defect Disease and Department of Pediatric Surgery, Guangzhou Women and Children's Medical Center, Guangzhou Medical University, Guangzhou 510623 Guangdong, China.
Yide MuProvincial Key Laboratory of Research in Structure Birth Defect Disease and Department of Pediatric Surgery, Guangzhou Women and Children's Medical Center, Guangzhou Medical University, Guangzhou 510623 Guangdong, China.
Zuyi MaProvincial Key Laboratory of Research in Structure Birth Defect Disease and Department of Pediatric Surgery, Guangzhou Women and Children's Medical Center, Guangzhou Medical University, Guangzhou 510623 Guangdong, China.
Gen ChenDepartment of Pharmacology, The First People's Hospital of Chenzhou, Chenzhou 423000 Hunan, China.
Yun ZhuProvincial Key Laboratory of Research in Structure Birth Defect Disease and Department of Pediatric Surgery, Guangzhou Women and Children's Medical Center, Guangzhou Medical University, Guangzhou 510623 Guangdong, China.
Yongwei HuangProvincial Key Laboratory of Research in Structure Birth Defect Disease and Department of Pediatric Surgery, Guangzhou Women and Children's Medical Center, Guangzhou Medical University, Guangzhou 510623 Guangdong, China.
Jiazhang ChenProvincial Key Laboratory of Research in Structure Birth Defect Disease and Department of Pediatric Surgery, Guangzhou Women and Children's Medical Center, Guangzhou Medical University, Guangzhou 510623 Guangdong, China.
Shenwei HuangProvincial Key Laboratory of Research in Structure Birth Defect Disease and Department of Pediatric Surgery, Guangzhou Women and Children's Medical Center, Guangzhou Medical University, Guangzhou 510623 Guangdong, China.
Qiuming HeProvincial Key Laboratory of Research in Structure Birth Defect Disease and Department of Pediatric Surgery, Guangzhou Women and Children's Medical Center, Guangzhou Medical University, Guangzhou 510623 Guangdong, China.
Lihua HuangProvincial Key Laboratory of Research in Structure Birth Defect Disease and Department of Pediatric Surgery, Guangzhou Women and Children's Medical Center, Guangzhou Medical University, Guangzhou 510623 Guangdong, China.
Xiaoyu ZuoProvincial Key Laboratory of Research in Structure Birth Defect Disease and Department of Pediatric Surgery, Guangzhou Women and Children's Medical Center, Guangzhou Medical University, Guangzhou 510623 Guangdong, China.
Fenjie LiProvincial Key Laboratory of Research in Structure Birth Defect Disease and Department of Pediatric Surgery, Guangzhou Women and Children's Medical Center, Guangzhou Medical University, Guangzhou 510623 Guangdong, China.
Jixiao ZengProvincial Key Laboratory of Research in Structure Birth Defect Disease and Department of Pediatric Surgery, Guangzhou Women and Children's Medical Center, Guangzhou Medical University, Guangzhou 510623 Guangdong, China. Electronic address: zengjixiao@163.com.
Huimin XiaProvincial Key Laboratory of Research in Structure Birth Defect Disease and Department of Pediatric Surgery, Guangzhou Women and Children's Medical Center, Guangzhou Medical University, Guangzhou 510623 Guangdong, China. Electronic address: xia-huimin@foxmail.com.
Yan ZhangProvincial Key Laboratory of Research in Structure Birth Defect Disease and Department of Pediatric Surgery, Guangzhou Women and Children's Medical Center, Guangzhou Medical University, Guangzhou 510623 Guangdong, China. Electronic address: yannizy@gwcmc.org.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

introductionEnteric neuronal injury significantly contributes to gastrointestinal motility disorders. While macrophages are known to interact closely with enteric neurons and modulate intestinal homeostasis, their specific impact on enteric neuronal survival and injury remains poorly understood.

objectiveThis study aimed to explore the potential contribution of macrophage-derived exosomes to enteric neuronal injury and investigate the underlying mechanisms.

methodsWe established a benzalkonium chloride (BAC)-induced enteric neuronal injury mouse model and assessed the effects of macrophage depletion on gastrointestinal motility and neuronal density. In vitro, enteric neurons were co-cultured with macrophages to examine the role of macrophage-derived exosomes in neuronal apoptosis. M1 macrophage-derived exosomes were isolated and characterized to elucidate potential mechanisms both in vitro and in vivo.

resultsMacrophage depletion partially rescued gastrointestinal dysmotility and enteric neuronal injury in BAC-treated mice. In vitro studies confirmed that M1 macrophage-derived exosomes promoted neuronal apoptosis. We identified matrix metalloproteinase 8 (MMP8) as enriched in M1 macrophage-derived exosomes, promoting the activation of the TGF-β signaling pathway and inducing neuronal apoptosis. Exosomes derived from M1 macrophages exacerbated neuronal injury in BAC mice, while inhibiting MMP8 partially mitigated neuronal injury and restored intestinal function. Additionally, elevated serum exosomal MMP8 levels were identified as a potential marker for Hirschsprung's disease, a typical gastrointestinal motility disorder.

conclusionM1 macrophages induce enteric neuronal injury via exosomal MMP8-mediated TGF-β signaling, highlighting a potential therapeutic target for gastrointestinal motility disorders.

Indexed as

Enteric Nervous SystemExosomesMacrophagesMatrix Metalloproteinase 8NeuronsTransforming Growth Factor betaAnimalsApoptosisBenzalkonium CompoundsDisease Models, AnimalGastrointestinal MotilityMaleMiceMice, Inbred C57BLSignal TransductionBenzalkonium CompoundsMatrix Metalloproteinase 8Transforming Growth Factor betaApoptosisEnteric neuronal injuryExosomesMacrophageMMP8

Identifiers

PMID41106724
PMCPMC13316501

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.