Evidence map›Paper›PMID 41106387›Full record

ArticleStem cell reports2025

GABA produced by multiple bone marrow cell types regulates hematopoietic stem and progenitor cells.

Cesi Deng, Adedamola Elujoba-Bridenstine, Ai Tang Song, Rylie M Ceplina, Casey J Ostheimer, Molly C Pellitteri Hahn, Cameron O Scarlett, Sahitya Saka, Xuan Pan, Owen J Tamplin

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cell reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

10 authors.

Cesi DengDepartment of Cell and Regenerative Biology, School of Medicine and Public Health, University of Wisconsin-Madison, Madison, WI, USA.
Adedamola Elujoba-BridenstineDepartment of Cell and Regenerative Biology, School of Medicine and Public Health, University of Wisconsin-Madison, Madison, WI, USA.
Ai Tang SongDepartment of Cell and Regenerative Biology, School of Medicine and Public Health, University of Wisconsin-Madison, Madison, WI, USA.
Rylie M CeplinaDepartment of Cell and Regenerative Biology, School of Medicine and Public Health, University of Wisconsin-Madison, Madison, WI, USA.
Casey J OstheimerDepartment of Cell and Regenerative Biology, School of Medicine and Public Health, University of Wisconsin-Madison, Madison, WI, USA.
Molly C Pellitteri HahnAnalytical Instrumentation Center, School of Pharmacy, University of Wisconsin-Madison, Madison, WI, USA.
Cameron O ScarlettAnalytical Instrumentation Center, School of Pharmacy, University of Wisconsin-Madison, Madison, WI, USA.
Sahitya SakaDepartment of Medical Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, WI, USA; Carbone Cancer Center, University of Wisconsin-Madison, Madison, WI, USA.
Xuan PanDepartment of Medical Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, WI, USA; Carbone Cancer Center, University of Wisconsin-Madison, Madison, WI, USA; Wisconsin Blood Cancer Research Institute, University of Wisconsin-Madison, Madison, WI, USA.
Owen J TamplinDepartment of Cell and Regenerative Biology, School of Medicine and Public Health, University of Wisconsin-Madison, Madison, WI, USA; Carbone Cancer Center, University of Wisconsin-Madison, Madison, WI, USA; Wisconsin Blood Cancer Research Institute, University of Wisconsin-Madison, Madison, WI, USA. Electronic address: tamplin@wisc.edu.

Funding

UW COMPREHENSIVE CANCER CENTER SUPPORTP30CA014520 · NCI · UNIVERSITY OF WISCONSIN-MADISON · PI Christian Capitini · 1985 to 2026
$142.6M
Mechanisms that Regulate Self-renewal and Proliferation in Hematopoietic Stem CellsR01HL146540 · NHLBI · UNIVERSITY OF WISCONSIN-MADISON · PI PAN, XUAN · 2019 to 2023
$1.9M
Multi-color Benchtop Flow CytometerS10RR025483 · NCRR · UNIVERSITY OF WISCONSIN-MADISON · PI BUSHMAN, WADE A · 2009 to 2009
$449k
Special BD LSR FortessaS10OD018202 · OD · UNIVERSITY OF WISCONSIN-MADISON · PI PATANKAR, MANISH S · 2014 to 2014
$351k
NCI NIH HHS P30 CA014520NCRR NIH HHS S10 RR025483NHLBI NIH HHS R01 HL146540NIH HHS S10 OD018202
6 · The paper itself

Abstract

Hematopoietic stem and progenitor cells (HSPCs) maintain homeostasis of the blood system by balancing proliferation and differentiation. Many extrinsic signals in the bone marrow (BM) microenvironment that regulate this balance are still unknown. We report gamma-aminobutyric acid (GABA) metabolite produced in the BM as a regulator of HSPCs. Deletion of the genes encoding the glutamate decarboxylase enzymes (Gad1 and Gad2) that produce GABA in either B lineages or endothelial cells (ECs) led to a slight reduction in BM HSPCs but not GABA levels. However, simultaneous blockade of GABA production from both hematopoietic cells and ECs resulted in a greater reduction of HSPCs and a significant reduction of BM GABA levels. Lower GABA levels in the BM altered the gene expression profile of HSPCs, with expression reduced for proliferation-associated genes and increased for B lineage genes. Our findings suggest GABA from multiple sources coordinates to regulate HSPC activity.

Indexed as

Bone Marrow Cellsgamma-Aminobutyric AcidHematopoietic Stem CellsAnimalsCell DifferentiationCell LineageCell ProliferationEndothelial CellsGlutamate DecarboxylaseMiceMice, Inbred C57BLgamma-Aminobutyric AcidGlutamate Decarboxylaseglutamate decarboxylase 1glutamate decarboxylase 2B cell lineage commitmentB cellsbone marrow nicheendothelial cellsGABAgamma-aminobutyric acidglutamate decarboxylasehematopoietic stem and progenitor cellsmicroenvironment

Identifiers

PMID41106387
PMCPMC12790740

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.